Recombinant Human MARCO Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7586-MA

R&D Systems, part of Bio-Techne
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7586-MA-050
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Key Product Details

Source

CHO

Accession #

Q9UEW3

Conjugate

Unconjugated

Applications

Binding Activity

View all MARCO Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human MARCO protein
Met79-Val520, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

44.7 kDa

SDS-PAGE

60-70 kDa, reducing conditions

Activity

Measured by its ability to bind fluorescein-conjugated E. coli Bioparticles. Sankala, M. et al. (2002) J. Biol. Chem. 277:33378.
The ED50 for this effect is 0.3-1.5 μg/mL.

Formulation, Preparation and Storage

7586-MA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MARCO

MARCO (macrophage receptor with collagenous structure), also known as SCARA2, is an 80 kDa type II transmembrane glycoprotein in the class A scavenger receptor family (1). Human MARCO consists of a 43 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 456 aa extracellular domain (ECD) that includes a stalk region, a collagen‑like region, and one SRCR domain (2). Within the ECD, human MARCO shares 69% aa sequence identity with mouse and rat MARCO. MARCO is constitutively expressed on the surface of splenic and lymph node macrophages (3). Its expression can be induced on Kupffer cells, alveolar macrophages, and glial cells by microbial infection, chemical irritants, and Th1 polarizing factors (4‑6). The SRCR domain mediates binding of MARCO to its various ligands, while the collagen‑like region mediates assembly into a disulfide-linked trimer (2, 3, 7). MARCO binds bacterial LPS and lipoteichoic acid, modified LDL, CpG oligonucleotides, UGRP1, silica, and TiO2 (3, 7‑10). It interacts in cis with the formyl peptide receptors FPR1 and FPRL1 on astrocytes and microglia (11). MARCO ligation promotes the production of inflammatory mediators by macrophages (8). MARCO mediated internalization of some ligands prevents their activation of cell surface TLR4 but enables their activation of intracellular TLR3 (12). MARCO contributes to the clearance of apoptotic cells and inhaled bacteria, mast cell mediated silicosis, and the amelioration of allergen or ozone induced lung inflammation (4, 13‑16). It is required for the organization of the splenic marginal zone and the interaction of splenic macrophages and B cells (17).

References

  1. Sheetal, A. et al. (2008) J. Immunotoxicol. 5:151.
  2. Elomaa, O. et al. (1998) J. Biol. Chem. 273:4530.
  3. Elomaa, O. et al. (1995) Cell 80:603.
  4. Dahl, M. et al. (2007) J. Clin. Invest. 117:757.
  5. Jozefowski, S. et al. (2005) J. Immunol. 175:8032.
  6. Braun, B.J. et al. (2011) J. Neuroinflamm. 8:11.
  7. Chen, Y. et al. (2006) J. Biol. Chem. 281:12767.
  8. Jozefowski, S. et al. (2006) J. Leukoc. Biol. 80:870.
  9. Bin, L.-H. et al. (2003) J. Immunol. 171:924.
  10. Hamilton, Jr., R.F. et al. (2006) J. Biol. Chem. 281:34218.
  11. Brandenburg, L.-O. et al. (2010) J. Neurochem. 113:749.
  12. Mukhopadhyay, S. et al. (2011) Blood 117:1319.
  13. Arredouani, M. et al. (2004) J. Exp. Med. 200:267.
  14. Rogers, N.J. et al. (2009) J. Immunol. 182:1982.
  15. Brown, J.M. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:43.
  16. Arredouani, M.S. et al. (2007) J. Immunol. 178:5912.
  17. Karlsson, M.C.I. et al. (2003) J. Exp. Med. 198:333.

Long Name

Macrophage Receptor with Collagenous Structure/Scavenger Receptor, Class A, Member 2

Alternate Names

SCARA2

Entrez Gene IDs

8685 (Human); 17167 (Mouse)

Gene Symbol

MARCO

UniProt

Q9UEW3

Additional MARCO Products

Product Documents for Recombinant Human MARCO Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Human MARCO Protein, CF

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