Recombinant Human MBL Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9086-MB

R&D Systems, part of Bio-Techne
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9086-MB-050
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Key Product Details

Source

HEK293

Accession #

CAB56121

Conjugate

Unconjugated

Applications

Bioactivity

View all MBL Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human MBL protein
Glu21-Ile248

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu21

Predicted Molecular Mass

24 kDa

SDS-PAGE

31-36 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Mannan is immobilized at 0.1 µg/mL (100 µL/well), the concentration of Recombinant Human MBL that produces 50% of the optimal binding response is approximately 25-150 ng/mL.

Reviewed Applications

Read 1 review rated 4 using 9086-MB in the following applications:

Scientific Data Images for Recombinant Human MBL Protein, CF

Recombinant Human MBL Protein Bioactivity

Recombinant Human MBL Protein Bioactivity

When Mannan is coated at 0.1 µg/mL (100 µL/well), Recombinant Human MBL (Catalog # 9086-MB) binds with a typical ED50 of 25-150 ng/mL.

Formulation, Preparation and Storage

9086-MB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MBL

Human mannose/mannan-binding lectin (MBL) is a 25-30 kDa secreted glycoprotein in the collectin family of pattern-recognition molecules (1, 2). Mature human MBL contains a cysteine-rich region, a collagen-like segment, and a C-type lectin domain that binds to neutral bacterial carbohydrates (3). Human MBL shares 61% amino acid sequence identity with mouse and rat MBL and carries variable post-translation modifications including O-glycosylation and proline and lysine hydroxylation (4). Its collagen-like region mediates MBL association into disulfide-stabilized trimers which further associate into complexes containing three or four copies of the basic trimer (4, 5). MBL is secreted by hepatocytes and opsonizes bacteria through interactions with microbial carbohydrates (5). Tetrameric complexes of MBL show greater carbohydrate binding capacity compared to the trimers (5). MBL multimers can associate with the serine proteases MASP-1, -2, and -3 and promote their cleavage of Complement Component C3 (5-8). Proteolytic cleavage of C3 triggers activation of the complement system and formation of the membrane attack complex, leading to destruction of opsonized bacteria (2). In addition, MBL binds to the scavenger receptor CD91 which mediates the clearance of apoptotic material (9).

References

  1. Scorza, M. et al. (2015) Clin. Chim. Acta 451:78.
  2. Matsushita, M. et al. (2013) Arch. Immunol. Ther. Exp. (Warsz.) 61:273.
  3. Ezekowitz, R.A.B. et al. (1988) J. Exp. Med. 167:1034.
  4. Jensen, P.H. et al. (2005) J. Biol. Chem. 280:11043.
  5. Teillet, F. et al. (2005) J. Immunol. 174:2870.
  6. Moller-Kristensen, M. et al. (2007) Int. Immunol. 19:141.
  7. Thiel, S. et al. (2000) J. Immunol. 165:878.
  8. Vorup-Jensen, T. et al. (2000) J. Immunol. 165:2093.
  9. Duus, K. et al. (2010) FEBS J. 277:4956.

Long Name

Mannose Binding Lectin

Alternate Names

COLEC1, Collectin-1, MBL2, MBL2D, MBP1

Entrez Gene IDs

4153 (Human)

Gene Symbol

MBL2

UniProt

CAB56121

Additional MBL Products

Product Documents for Recombinant Human MBL Protein, CF

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Product Specific Notices for Recombinant Human MBL Protein, CF

For research use only

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