Recombinant Human MGAT4A His-tag, CF

R&D Systems, part of Bio-Techne | Catalog # 11496-GT

Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A
R&D Systems, part of Bio-Techne
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11496-GT-025
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Key Product Details

Source

CHO

Accession #

Q9UM21

Conjugate

Unconjugated

Applications

Enzyme Activity

View all MGAT4A Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human MGAT4A protein
Leu93-Asn535, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu93

Predicted Molecular Mass

52 kDa

SDS-PAGE

54-60 kDa, under reducing conditions.

Activity

Measured by its ability to transfer N-acetyl-alpha -D-glucosamine from UDP-N-Acetyl-alpha -D-glucosamine to glycan Cy5-Fuc labeled N2f.
Able to convert >85% of the substrate glycan N2f, as measured under the described conditions.

Scientific Data Images for Recombinant Human MGAT4A His-tag, CF

Recombinant Human MGAT4A His-tag Protein Enzyme Activity Diagram.

Recombinant Human MGAT4A His-tag Protein (Catalog # 11496-GT) catalyzes a beta1-4GlcNAc linkage to the alpha1-3Man arm of N-glycans.

Recombinant Human MGAT4A His-tag Protein Enzyme Activity.

Recombinant Human MGAT4A His-tag Protein (Catalog # 11496-GT) activity was measured by its ability to transfer N-acetyl-alpha -D-glucosamine from UDP-N-acetyl-alpha -D-glucosamine to glycan Cy5-Fuc labeled N2f (GL304). Following treatment, a mobility shift was observed via SDS-PAGE gel.

Recombinant Human MGAT4A His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human MGAT4A His-tag Protein (Catalog # 11496-GT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-60 kDa, under reducing conditions.

Formulation, Preparation and Storage

11496-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: MGAT4A

Recombinant human Alpha-1,3-mannosyl-glycoprotein 4-beta -N-acetylglucosaminyltransferase A (MGAT4A), also known as GlcNAc-T IVa , is a metal-dependent, golgi single-pass type II membrane protein that contains an N-terminal transmembrane region, a catalytic domain, and a C-terminal carbohydrate binding module that regulates its catalytic activity (1, 2). MGAT4A is one of several human MGAT enzymes involved in the initiation and synthesis of N-glycans, with each enzyme having unique specificity and preferences (3). MGAT4 has two human isozymes that catalyze formation of the beta1-4 GlcNAc branch in the  alpha1-3 Mannose arm of N-glycans (4-6). The two isozymes have different expression profiles in tissues and cancer cell lines , with MGAT4A expression within gastrointestinal tissues such as the pancreas (2, 4, 5). Loss of MGAT4A in mouse models has been shown to induce type 2 diabetes (9,10). Expression and activity of MGAT4A is particularly important during differentiation and oncogenesis (7, 8) including within choriocarcinoma, invasive mole, and placental site trophoblastic tumors (11-13). The discovery of MGAT4A modulators may lead to new therapeutics for the treatment of these diseases (10). MGAT4A may also be useful as a tool for the glycoengineering of complex N-glycans. The activity of MGAT4A was demonstrated using a fluorescent gel shift assay.

References

  1. Oka, N. et al. (2022) Glycobiology. 32:1153.
  2. Nagae, M. et. al. (2022) Commun. Biol. 5:695.
  3. Brockhausen, I. et. al. (1988) Biochemie 70:1521.
  4. Yoshida, A. et. al. (1999) Glycobiology. 9:303.
  5. Oguri, S. et. al. (2006) Glycoconj. J. 23:473.
  6. Osada, N. et. al. (2022) J. Biol. Chem. 298:102400.
  7. Nishikawa, A. et. al. (1990) Biochim. Biophys. Acta. 1035:313.
  8. Nakao, H. et. al. (1990) Biochem. Biophys. Res. Commun. 172:1260.
  9. Ohtsubo, K. et. al. (2005) Cell. 123:1307.
  10. Ohtsubo, K. et. al. (2011) Nat. Med. 17:1067.
  11. Mizuochi, T. et. al. (1983) J. Biol. Chem. 258:14126.
  12. Endo, T. et. al. (1987) Cancer Res. 47:5242.
  13. Niimi, K. et. al. (2012) Br. J. Cancer. 107:1969.

Long Name

alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A

Alternate Names

EC 2.4.1.145, GlcNAc-T IVa, GnT-4a, GNT-IV, GNT-IVA, N-Acetylglucosaminyltransferase IVa

Entrez Gene IDs

11320 (Human); 269181 (Mouse); 367252 (Rat)

Gene Symbol

MGAT4A

UniProt

Q9UM21 (Human)

Additional MGAT4A Products

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Product Specific Notices for Recombinant Human MGAT4A His-tag, CF

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