Recombinant Human MMP-17 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7796-MP

R&D Systems, part of Bio-Techne
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7796-MP-010
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Key Product Details

Source

NS0

Accession #

Q9ULZ9

Conjugate

Unconjugated

Applications

Enzyme Activity

View all MMP-17 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human MMP-17 protein
Ala39-Ser531 (Arg125Pro) & (Ala182Thr), with a C-terminal 6-His tag

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

N-terminal Sequence Analysis

Ala39

Predicted Molecular Mass

47 kDa

SDS-PAGE

56-65 kDa, reducing conditions

Activity

Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2 (Catalog # ES003).
The specific activity is >150 pmol/min/μg, as measured under the described condition.

Formulation, Preparation and Storage

7796-MP
Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl, CaCl2 and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: MMP-17

Matrix metalloproteinase 17 (MMP‑17) is a zinc metalloproteinase that is an integral membrane protein (1). It belongs to the membrane‑type MMP (MT‑MMP) subfamily, and is also known as MT4‑MMP. It is associated with the cell membrane by a glycosylphosphatidylinositol (GPI) anchor (1). It has the potential to function as a pro‑tumor necrosis factor‑a  (TNF‑a) convertase (2). Unlike MMP‑14 (MT1‑MMP), it is a poor activator of pro‑MMP‑2. MMP‑17 is relatively poor at digesting components of the extracellular matrix, but does cleave fibrinogen and fibrin (2). MMP‑17 is known to activate the aggrecanase ADAMTS‑4 through proteolysis (3). MMP‑17 is expressed in many tissues with the highest levels seen in the brain, colon, ovary, testis, and kidney papilla (4). It has been detected in several human cancers, including gliomas, prostate carcinomas, and breast carcinomas (5). Recombinant Human MMP‑17 was expressed without its GPI anchoring sequence, resulting in its secretion as a soluble protein.

References

  1. Itoh, Y. et al. (1999) J. Biol. Chem. 274:34260.
  2. English, W. R. et al. (2000) J. Biol. Chem. 275:14046.
  3. Gao, G. et al. (2004) J. Biol. Chem. 279:10042.
  4. Srichai, M. B. et al. (2011) PLoS ONE 6:e17099.
  5. Sohail, A. et al. (2008) Cancer Metastasis Rev.  27:289.

Long Name

Matrix Metalloproteinase 17

Alternate Names

MMP17, MT4-MMP

Entrez Gene IDs

4326 (Human); 23948 (Mouse); 288626 (Rat)

Gene Symbol

MMP17

UniProt

Q9ULZ9 (Human)

Additional MMP-17 Products

Product Documents for Recombinant Human MMP-17 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human MMP-17 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

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