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Recombinant Human MSP/MST1 (CHO-expressed) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 352-MCH

R&D Systems, part of Bio-Techne
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352-MCH-010

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human MSP/MST1 protein
Gln19-Arg483 ( alpha-chain) & Val484-Gly711 ( beta-chain)

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln19 predicted ( alpha-chain) & Val484 ( beta-chain)

Predicted Molecular Mass

53 kDa ( alpha-chain), 25 kDa ( beta-chain)

SDS-PAGE

85 kDa, non-reducing conditions

Activity

Measured by its ability to activate MSP R/Ron in MDA-MB-453 or T47D human breast cancer cells.
80 ng/mL of the recombinant human MSP significantly induces phosphorylation of MSP R/Ron measured by the Human Phospho-MSP R/Ron DuoSet® IC Kit (Catalog # DYC1947).

Measured by its binding ability in a functional ELISA.
When Recombinant Human MSPR/Ron Protein (Catalog # 1947-MS) is represent at 100 ng/mL (100 µg/mL), The concentration of Recombinant Human MSP/MST1 that produces 50% optimal binding response is found to be 8-40 ng/mL.

Formulation, Preparation and Storage

352-MCH
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSP/MST1

Macrophage stimulating protein (MSP), also known as HGF-like protein, and scatter factor-2, is a member of the HGF family of growth factors (1). MSP is secreted as an inactive single chain precursor (pro-MSP) that contains a PAN/APPLE-like domain, four kringle domains, and a peptidase S1 domain which lacks enzymatic activity (2). Human MSP shares 79% aa sequence identity with mouse MSP, and 44% aa sequence identity with human HGF. Pro-MSP is secreted by hepatocytes under the positive and negative control of CBP in complex with either HNF-4 or RAR, respectively (3). Circulating pro-MSP is proteolytically cleaved in response to tissue injury to yield a biologically active disulfide linked heterodimer consisting of a 45 - 62 kDa alpha and a 25 - 35 kDa beta chain (4, 5). Pro-MSP can be activated by MT-SP1, a transmembrane protease that is expressed on macrophages and is upregulated in many cancers (6). Heterodimeric MSP as well as the isolated beta chain bind to MSP R/Ron with high-affinity; however, only heterodimeric MSP can induce receptor dimerization and signaling (7, 8). MSP induces macrophage and keratinocyte proliferation and osteoclast activation (9, 10). It also inhibits LPS- or IFN-induced iNOS and IL-12 expression by macrophages, and prevents apoptosis of epithelial cells separated from the ECM (11, 12).

References

  1. Wang, M.-H. et al. (2002) Scand. J. Immunol.56:545.
  2. Han, S. et al. (1991) Biochemistry 30:9768.
  3. Muraoka, R.S. et al. (1999) Endocrinology 140:187.
  4. Wang, M.-H. et al. (1996) J. Clin. Invest. 97:720.
  5. Nanney, L.B. et al. (1998) J. Invest. Dermatol. 111:573.
  6. Bhatt, A.S. et al. (2007) Proc. Natl. Acad. Sci. 104:5771.
  7. Wang, M.-H. et al. (1997) J. Biol. Chem. 272:16999.
  8. Danilkovitch, A. et al. (1999) J. Biol. Chem. 274:29937.
  9. Wang, M.-H. et al. (1996) Exp. Cell Res. 226:39.
  10. Kurihara, N. et al. (1998) Exp. Hematol. 26:1080.
  11. Morrison, A.C. et al. (2004) J. Immunol. 172:1825.
  12. Liu, Q.P. et al. (1999) J. Immunol. 163:6606.

Long Name

Macrophage Stimulating Protein/Macrophage Stimulating 1 [Hepatocyte Growth Factor-like]

Alternate Names

HGFL, MST1, SF2

Entrez Gene IDs

4485 (Human); 15235 (Mouse); 24566 (Rat)

Gene Symbol

MST1

Additional MSP/MST1 Products

Product Documents for Recombinant Human MSP/MST1 (CHO-expressed) Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human MSP/MST1 (CHO-expressed) Protein, CF

For research use only

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