Recombinant Human MSP/MST1 (Cys672Ala) Protein
R&D Systems, part of Bio-Techne | Catalog # 4306-MS
Key Product Details
Source
CHO
Accession #
Structure / Form
Disulfide-linked heterodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human MSP/MST1 protein
Gln19-Arg483 (alpha) & Val484-Gly711 (Cys672Ala) (beta)
Gln19-Arg483 (alpha) & Val484-Gly711 (Cys672Ala) (beta)
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gln19 & Val484
Predicted Molecular Mass
53 kDa ( alpha-chain), 25 kDa ( beta-chain)
SDS-PAGE
60 kDa and 30 kDa, reducing conditions
Activity
Measured by its ability to activate MSP R/Ron in MDA-MB-453 or T47D human breast cancer cells.
80 ng/mL of the recombinant human MSP (C672A) significantly induces phosphorylation of MSP R/Ron measured by the Human Phospho-MSP R/Ron DuoSet® IC Kit (Catalog # DYC1947).
Measured by its binding ability in a functional ELISA.
When Recombinant Human MSPR/Ron Protein (Catalog #1947-MS) is represent at 100 ng/mL (100 µg/mL), The concentration of Recombinant Human MSP/MST1 that produces 50% optimal binding response is found to be 4-32 ng/mL.
80 ng/mL of the recombinant human MSP (C672A) significantly induces phosphorylation of MSP R/Ron measured by the Human Phospho-MSP R/Ron DuoSet® IC Kit (Catalog # DYC1947).
Measured by its binding ability in a functional ELISA.
When Recombinant Human MSPR/Ron Protein (Catalog #1947-MS) is represent at 100 ng/mL (100 µg/mL), The concentration of Recombinant Human MSP/MST1 that produces 50% optimal binding response is found to be 4-32 ng/mL.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 4306-MS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 4306-MS/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: MSP/MST1
Macrophage stimulating protein (MSP), also known as HGF-like protein, and scatter factor-2, is a member of the HGF family of growth factors (1). MSP is secreted as an inactive single chain precursor (pro-MSP) that contains a PAN/APPLE-like domain, four kringle domains, and a peptidase S1 domain which lacks enzymatic activity (2). Human MSP shares 79% aa sequence identity with mouse MSP and 44% aa sequence identity with human HGF. Pro-MSP is secreted by hepatocytes under the positive and negative control of CBP in complex with either HNF-4 or RAR, respectively (3). Circulating pro-MSP is proteolytically cleaved in response to tissue injury to yield biologically active disulfide linked heterodimers consisting of a 45 - 62 kDa alpha and a 25 - 35 kDa beta chain (4, 5). Pro-MSP can be activated by MT-SP1, a transmembrane protease that is expressed on macrophages and is upregulated in many cancers (6). Heterodimeric MSP, as well as the isolated beta chain, binds to MSP R/Ron with high-affinity, although only heterodimeric MSP can induce receptor dimerization and signaling (7, 8). MSP induces macrophage and keratinocyte proliferation and osteoclast activation (9, 10). It also inhibits LPS- or IFN-induced iNOS and IL-12 expression by macrophages and prevents apoptosis of epithelial cells separated from the ECM (11, 12). The substitution of cysteine 672 (in the beta chain) with alanine significantly increases the bioactivity of recombinant MSP, apparently by limiting incorrect disulfide bond formation between the alpha and beta chains (13).
References
- Wang, M.-H. et al. (2002) Scand. J. Immunol.56:545.
- Han, S. et al. (1991) Biochemistry 30:9768.
- Muraoka, R.S. et al. (1999) Endocrinology 140:187.
- Wang, M.-H. et al. (1996) J. Clin. Invest. 97:720.
- Nanney, L.B. et al. (1998) J. Invest. Dermatol. 111:573.
- Bhatt, A.S. et al. (2007) Proc. Natl. Acad. Sci. 104:5771.
- Wang, M.-H. et al. (1997) J. Biol. Chem. 272:16999.
- Danilkovitch, A. et al. (1999) J. Biol. Chem. 274:29937.
- Wang, M.-H. et al. (1996) Exp. Cell Res. 226:39.
- Kurihara, N. et al. (1998) Exp. Hematol. 26:1080.
- Morrison, A.C. et al. (2004) J. Immunol. 172:1825.
- Liu, Q.P. et al. (1999) J. Immunol. 163:6606.
- Wahl, R.C. et al. (1997) J. Biol. Chem. 272:15053.
Long Name
Macrophage Stimulating Protein/Macrophage Stimulating 1 [Hepatocyte Growth Factor-like]
Alternate Names
HGFL, MST1, SF2
Gene Symbol
MST1
UniProt
Additional MSP/MST1 Products
Product Documents for Recombinant Human MSP/MST1 (Cys672Ala) Protein
Product Specific Notices for Recombinant Human MSP/MST1 (Cys672Ala) Protein
For research use only
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