Recombinant Human MUC-1 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10332

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

P15941.3

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all MUC-1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human MUC-1 protein
Human MUC-1
(Ser24-Ser380)
Accession # P15941.3		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

62 kDa

SDS-PAGE

168-195 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Biotinylated Recombinant Human MUC-1 Fc Chimera Avi-tag (Catalog # AVI10332) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Siglec-9 Fc Chimera (Catalog # 1139-SL) binds with an ED50 of 0.25-2 µg/mL.

Scientific Data Images for Recombinant Human MUC-1 Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant MUC-1 Fc Chimera Avi-tag Protein Binding Activity.

When Biotinylated Recombinant Human MUC-1 Fc Chimera Avi-tag (Catalog # AVI10332) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Siglec-9 Fc Chimera (1139-SL) binds with an ED50 of 0.25-2 µg/mL.

Biotinylated Recombinant Human MUC-1 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human MUC-1 Fc Chimera Avi-tag (Catalog # AVI10332) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 168-195 kDa and 320-380 kDa, respectively.

Formulation, Preparation and Storage

AVI10332
Formulation Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: MUC-1

MUC-1 (Mucin-1) is a type 1 transmembrane glycoprotein that is normally expressed on the apical surface of most epithelial cells (1-2). It is known to be overexpressed by various human carcinomas and is shed into circulation (2). The extracellular domain is made up of tandem repeats (TRs) of 20 amino acid (aa) each, with each TR containing five potential O-glycosylation sites (3). The number of TRs vary between 25-100, depending on the allele (3). Within the mature region including 16 TRs (residues 24-380), human MUC-1 shares 30% aa sequence identity with mouse and rat MUC-1. It has been reported that high expression level of MUC-1 generally correlates with increased mortality rates (4). In addition, MUC-1 is aberrantly underglycosylated on cancer cells with short and sialylated O-linked glycans in contrast to the long, branched chain seen in normal epithelial cells (4-7). It has been demonstrated that MUC-1 can interact with E-selectin and ICAM-1 to mediate firm adhesion of circulating tumor cells and subsequent extravasation in the metastatic adhesion cascade (4). Furthermore, MUC-1 can modulate the tumor immunological microenvironment through engagement of Siglec-9 by inducing the recruitment of beta-catenin to the cytoplasmic tail of MUC-1, increasing the expression of PD-L1 by macrophages, and activating the MEK-ERK pathway (5,6). MUC-1 can also interact with Galectin-3 to promote EGFR activation thus regulating EGFR-associated tumorigenesis and cancer progression (7). Our Avi-tag Biotinylated human MUC-1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Rughetti, A. et al. (2005) J. Immunol. 174:7764.
  2. Engelstaedter, V. et al. (2012) BMC Cancer 12:600.
  3. Taylor-Papadimitriou, J. et al. (1999) Biochim. Biophys. Acta 1455:301.
  4. Geng, Y. et al. (2012) Front Oncol. 2:76.
  5. Tanida, S. et al. (2013) J Biol Chem. 288:31842.
  6. Beatson, R. et al. (2016) Nat Immunol. 17:1273.
  7. Piyush, T. et al. (2017) Cell Death Differ. 24:1937.

Long Name

Mucin 1, Cell Surface-associated

Alternate Names

CD227, Episialin, H23AG, KL-6, Mucin-1, PEM, PEMT

Entrez Gene IDs

4582 (Human)

Gene Symbol

MUC1

UniProt

P15941.3

Additional MUC-1 Products

Product Documents for Recombinant Human MUC-1 Fc Chimera Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human MUC-1 Fc Chimera Avi-tag Protein, CF

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