Recombinant Human NELL1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5487-NL

R&D Systems, part of Bio-Techne
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5487-NL-050
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Key Product Details

Source

CHO

Accession #

Q92832

Conjugate

Unconjugated

Applications

Bioactivity

View all NELL1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human NELL1 protein
Arg17-Asn810, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg17 (33%) & Phe22 (66%)

Predicted Molecular Mass

88.6 kDa & 88.1 kDa

SDS-PAGE

100-130 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of C3H10T1/2 mouse embryonic fibroblast cells.
The ED50 for this effect is 1‑4 μg/mL.

Formulation, Preparation and Storage

5487-NL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 300 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NELL1

NELL1 (neural EGF‑like like protein 1) is an approximately 140 kDa modular glycoprotein that plays an important role in bone physiology (1). NELL1 contains an N‑terminal Laminin G‑like domain and three vWF‑C domains interspersed with five tandem EGF‑like domains (2). Mature human NELL1 shares 93% aa identity with mouse and rat NELL1. Alternative splicing of human NELL1 generates a short isoform that lacks the fourth EGF‑like domain. NELL1 can be retained in the cytosol where it interacts with ARP3 (apoptosis‑related protein 3) and becomes phosphorylated by PKC (3, 4). NELL1 is secreted as an approximately 400 kDa noncovalent homotrimer of heavily glycosylated subunits (5). It is expressed in bone and in select B cell lines (6, 7). NELL1 promotes the osteogenic differentiation of adipose‑derived stromal/stem cells and inhibits adipogenic differentiation (8). It does not promote osteoblastic differentiation from myoblasts, but it does enhance the activity of BMP‑2 in that function (9). NELL1 interacts directly with osteoblasts via Integrin alpha3 beta1 (10, 11) and promotes osteoblast differentiation and mineralization (3, 12, 13). It synergizes with BMP‑2 to increase phosphate uptake through the transporters Pit1 and Pit2 in pre‑osteoblasts (14). In vivo, NELL1 expression in the cranium is localized at sites of suture closure (6). Its over‑expression induces multiple abnormalities in cranial development including premature suture closure (craniosyntosis) and overlapping sutures (12, 13). Experimental engrafting demonstrates the ability of NELL1 to promote new bone formation and the healing of calvarial defects (13, 15).

References

  1. Zhang, X. et al. (2010) J. Dent. Res. 89:865.
  2. Watanabe, T.K. et al. (1996) Genomics 38:273.
  3. Zou, X. et al. (2011) FEBS Lett. 585:2410.
  4. Kuroda, S. and K. Tanizawa (1999) Biochem. Biophys. Res. Commun. 265:752.
  5. Kuroda, S. et al. (1999) Biochem. Biophys. Res. Commun. 265:79.
  6. Ting, K. et al. (1999) J. Bone Miner. Res. 14:80.
  7. Luce, M.J. and P.D. Burrows (1999) Gene 231:121.
  8. James, A.W. et al. (2012) Stem Cells Dev. 21:2170.
  9. Cowan, C.M. et al. (2007) J. Bone Miner. Res. 22:918.
  10. Hasebe, A. et al. (2012) FEBS Lett. 586:2500.
  11. Shen, J. et al. (2012) J. Cell. Biochem. 113:3620.
  12. Zhang, X. et al. (2002) J. Clin. Invest. 110:861.
  13. Aghaloo, T. et al. (2006) Am. J. Pathol. 169:903.
  14. Cowan, C.M. et al. (2012) Biochem. Biophys. Res. Commun. 422:351.
  15. Xue, J. et al. (2011) Bone 48:485.

Long Name

NEL-like 1

Alternate Names

IDH3GL

Entrez Gene IDs

4745 (Human); 338352 (Mouse)

Gene Symbol

NELL1

UniProt

Q92832

Additional NELL1 Products

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Product Specific Notices for Recombinant Human NELL1 Protein, CF

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