Recombinant Human NELL2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8946-NL

R&D Systems, part of Bio-Techne
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8946-NL-050
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Key Product Details

Source

NS0

Accession #

Q99435

Conjugate

Unconjugated

Applications

Bioactivity

View all NELL2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human NELL2 protein
Leu22-Leu816, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu22

Predicted Molecular Mass

90 kDa

SDS-PAGE

100-125 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of C3H10T1/2 mouse embryonic fibroblast cells.
The ED50 for this effect is 0.3-1.8 μg/mL.

Formulation, Preparation and Storage

8946-NL
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Citrate, NaCl and EDTA.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NELL2

NELL2 is an approximately 140 kDa secreted glycoprotein that exhibits several actions during nervous system development. NELL2 associates into a homotrimer and contains one Laminin G domain, one vWF-C domain, and six EGF-like domains, followed by two more vWF-C domains (1). Mature human NELL2 shares 94% aa sequence identity with mouse and rat NELL2. Alternative splicing generates a cytosolic isoform that associates with PKC beta 1 and can inhibit PKC signaling (2). NELL2 is widely expressed in neurons of the central and peripheral nervous systems, particularly in the hippocampus, hypothalamus, cerebral cortex, and cerebellar cortex (3-5). It is selectively expressed in VGAT1+ or VGAT2+ glutamatergic neurons (4, 6). During development, NELL2 is expressed in select neural crest cells, retinal ganglion cells, and hypaxial muscle precursor cells of the dermomyotome (7). It promotes the differentiation and migration of both motor and sensory neurons (8), enhances neuronal survival (9), and promotes neuronal aggregation by inducing N-Cadherin up-regulation (10). NELL2 supports the release of GnRH from the hypothalamus which is important for the onset of female puberty (5, 6). NELL2 is additionally expressed in renal tubules but is down-regulated in renal cancer (11).

References

  1. Watanabe, T.K. et al. (1996) Genomics 38:273.
  2. Lee, D.Y. et al. (2014) Biochem. Biophys. Res. Commun. 454:459.
  3. Oyasu, M. et al. (2000) Brain Res. Mol. Brain Res. 76:151.
  4. Jeong, J.K. et al. (2008) Mol. Cells 26:186.
  5. Zhou, S.S. and P. Li (2014) Genet. Mol. Res. 13:6672.
  6. Ha, C.M. et al. (2008) Neuroendocrinology 88:199.
  7. Nelson, B.R. et al. (2002) Mech. Dev. 119S:S11.
  8. Nelson, B.R. et al. (2004) Dev. Biol. 270:322.
  9. Aihara, K. et al. (2003) Mol. Brain Res. 116:86.
  10. Kim, D.H. et al. (2014) PLoS One 9:e85898.
  11. Nakamura, R. et al. (2015) Cancer Sci. 106:656.

Long Name

NEL-like 2

Alternate Names

Mel91, NRP2

Entrez Gene IDs

4753 (Human); 54003 (Mouse); 81734 (Rat)

Gene Symbol

NELL2

UniProt

Q99435 (Human)

Additional NELL2 Products

Product Documents for Recombinant Human NELL2 Protein, CF

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Product Specific Notices for Recombinant Human NELL2 Protein, CF

For research use only

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