Recombinant Human NETO1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11103-NO

R&D Systems, part of Bio-Techne
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11103-NO-050
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Key Product Details

Source

CHO

Accession #

Q8TDF5.1

Conjugate

Unconjugated

Applications

Bioactivity

View all NETO1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human NETO1 protein
Thr23-Val345, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr23

Predicted Molecular Mass

37 kDa

SDS-PAGE

34-48 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human NETO1 His-tag Protein is immobilized at 4.00 µg/mL (100 µL/well), Recombinant Human APP695+1 Fc Chimera binds with an ED50 of 0.200-1.20 μg/mL.

Scientific Data Images for Recombinant Human NETO1 His-tag Protein, CF

Recombinant Human NETO1 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human NETO1 His-tag Protein (Catalog # 11103-NO) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-48 kDa.

Formulation, Preparation and Storage

11103-NO
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NETO1

Neuropilin and tolloid-like protein 1 (NETO1), also known as Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 (BTCL1), is a type I transmembrane protein that is expressed in the brain and retina (1). The 511 amino acid (aa) residue mature human NETO1 contains a 322 aa extracellular domain (ECD) with two CUB domains and one class A LDL-receptor segment, a 21 aa transmembrane domain, and a 168 aa cytosol domain (1). Three human NETO1 splice isoforms exist, including a secreted soluble variant that shows an Asp-to-Glu transition at residue 157 followed by a premature truncation (1). The ECD of human NETO1 shares 97% and 98% aa identity with that of mouse and canine NETO1, respectively. NETO1 is expressed in the human brain and retina (1, 2). It increases the rate of GluK1 receptor desensitization and is a candidate NMDAR auxiliary subunit regulating channel activities and synaptic transmission (3, 4). NETO1 expression is associated with metastasis and poor survival in epithelial ovarian cancer (5).

References

  1. Stöhr, H. et al. (2002) Gene 286:223.
  2. Tomita, S. and P.E. Castillo (2012) J. Physiol. 590:2217.
  3. Copits, B.A. et al. (2011) J. Neurosci. 31:7334.
  4. Ng, D. et al. (2009) PloS Biol. 7:e41.
  5. Xu, Y. et al. (2020) J. Cell. Mol. Med. 24:9114.

Long Name

Neuropilin [NRP] And Tolloid [TLL]-like 1

Alternate Names

BCTL1, BTCL1

Entrez Gene IDs

81832 (Human); 246317 (Mouse); 307206 (Rat)

Gene Symbol

NETO1

UniProt

Q8TDF5.1

Additional NETO1 Products

Product Documents for Recombinant Human NETO1 His-tag Protein, CF

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Product Specific Notices for Recombinant Human NETO1 His-tag Protein, CF

For research use only

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