Recombinant Human NETO2 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10975-NO

R&D Systems, part of Bio-Techne
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10975-NO-050
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Key Product Details

Source

NS0

Accession #

Q8NC67.1

Conjugate

Unconjugated

Applications

Bioactivity

View all NETO2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human NETO2 protein
Ile23-Lys345, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile23

Predicted Molecular Mass

37 kDa

SDS-PAGE

32-45 kDa, reducing conditions.

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human NETO2 His-tag, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.

Scientific Data Images for Recombinant Human NETO2 His-tag Protein, CF

Recombinant Human NETO2 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human NETO2 His-tag Protein (Catalog # 10975-NO) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 32-45 kDa.

Formulation, Preparation and Storage

10975-NO
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NETO2

NETO2, or Neuropilin and tolloid-like protein 2, is a type 1 transmembrane protein belonging to the CUB (for complement C1r/C1s, Ueqf, Bmp1) domain and LDLa-containing protein family that modulates the neuronal kainate receptors (KARs) (1-3). It is mainly expressed in neural tissues but was also reported to be expressed in several non-neural tissues and its expression has been associated with various cancers such as renal, lung, colon, cervical and colorectal cancers (4-6). Mature human NETO2 is composed of a 325 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 157 aa cytoplasmic region. Within the ECD, human NETO2 shares 98% aa identity with mouse and rat NETO2. It functions as an accessory subunit of neuronal kainate-sensitive glutamate receptors, GRIK2 and GRIK3 regulating channel activities and synaptic transmission (7).

References

  1. Fedorova, M.S. et al. (2020) Front. Genet. 11:594933. doi: 10.3389/fgene.2020.594933.
  2. Stohr, H. et al. (2002) Gene 286:223.
  3. Straub, C. et al. (2011) J. Neurosci. 31:8078.
  4. Li, Y. et al. (2019) Cancer Manag. Res. 11:5147.
  5. Niu, O. et al. (2012) Genetika. 48:506.
  6. Xu, J. et al. (2021) Int. J. Biol. Sci. 17:259.
  7. Griffith T. et al. (2015) J. Physiol. 593:4815.

Long Name

Neuropilin [NRP] And Tolloid [TLL]-like 2

Alternate Names

BTCL2, NEOT2

Entrez Gene IDs

81831 (Human); 74513 (Mouse); 307757 (Rat)

Gene Symbol

NETO2

UniProt

Q8NC67.1

Additional NETO2 Products

Product Documents for Recombinant Human NETO2 His-tag Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Human NETO2 His-tag Protein, CF

For research use only

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