Recombinant Human NgR3/NgRH2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8439-NG

R&D Systems, part of Bio-Techne
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8439-NG-025
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Key Product Details

Source

HEK293

Accession #

Q86UN2

Conjugate

Unconjugated

Applications

Bioactivity

View all NgR3/NgRH2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human NgR3/NgRH2 protein
Cys25-Ala419, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys25

Predicted Molecular Mass

45 kDa

SDS-PAGE

56-65 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human APP695 is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Human NgR3/NgRH2 that produces 50% optimal binding response is 0.05-0.25 μg/mL.

Formulation, Preparation and Storage

8439-NG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NgR3/NgRH2

NgR3/NgRH2 (also known as reticulon-4 receptor-Like 1), along with the related NgR1 and NgR2, is a member of the Nogo66 receptor (NgR) family. NgR proteins are expressed primarily by neurons in the central and peripheral mammalian nervous system. NgR proteins play an essential role in mediating axon growth inhibition induced by the structurally distinct proteins Nogo, myelin associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (OMgp) (1, 2).These proteins belong to the family of myelin-associated inhibitors (MAIs). NgR proteins are composed of 8 leucine-rich repeats (LRRs) flanked by N-terminal and C-terminal leucine-rich capping domains and are attached at the cell surface by a C-terminal glycosylphosphatidylinositol (GPI) anchor (3). Because NgR proteins have no transmembrane domain, they must be associated with membrane proteins in order to transduce cell signaling (2). Both NGF R/TNFRSF16 (p75) and TROY/TNFRSF19 have been identified as NgR1-associated components (4, 5). Human NgR3 shares 88% and 86% amino acid sequence identity with mouse and rat NgR3 respectively. NgR1 and NgR3 serve as receptors for chondroitin sulfate proteoglycans (CSPGs) (6). NgR3 may function as a NgR1 co-receptor for Nogo66 (7). CSPGs and MAIs are known to stabilize neuronal structure and influence the growth and guidance of developing neurons (8). Therefore, NgR proteins are part of a multicomponent receptor system implicated in pathways that limit neuronal growth and increase the structural stability of synapses.

References

  1. GrandPre, T. et al. (2002) Nature 417:547.
  2. Wang, K.C. et al. (2002) Nature 420:74.
  3. Fournier, A.E. et al. (2001) Nature 409:341.
  4. Shao, Z. et al. (2005) Neuron 45:353.
  5. Park, J. B. et al. (2005) Neuron 45:345.
  6. Dickendesher, T.L. et al. (2012) Nat. Neurosci. 15:703.
  7. Zang, G. et al. (2011) J. Genet. Genomics 38:515.
  8. Kantor, D.B. et al. (2004) Neuron 44:961.

Long Name

Nogo-66 Receptor-related Protein 3/Nogo-66 Receptor Homolog 2/Reticulon-4 Receptor-like 1

Alternate Names

NgRH2, RTN4RL1

Entrez Gene IDs

146760 (Human); 237847 (Mouse)

Gene Symbol

RTN4RL1

UniProt

Q86UN2

Additional NgR3/NgRH2 Products

Product Documents for Recombinant Human NgR3/NgRH2 Protein, CF

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Product Specific Notices for Recombinant Human NgR3/NgRH2 Protein, CF

For research use only

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