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Recombinant Human Ninjurin-1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9717-NJ

R&D Systems, part of Bio-Techne
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9717-NJ-050

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Product Specifications

Source

E. coli-derived human Ninjurin-1 protein
Asp2-Val81
with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp2 & Ser3

Predicted Molecular Mass

9 kDa

SDS-PAGE

10 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9717-NJ
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ninjurin-1

Ninjurin-1 (nerve injury-induced protein 1) is a 20-22 kDa member of the Ninjurin family of transmembrane (TM) proteins (1, 2). It is expressed by Schwann cells, neurons and hepatocytes and participates in intercellular homophilic binding during nerve regeneration. Human Ninjurin-1 is 152 amino acids in length. It has an unusual membrane orientation. There is an 80 amino acid (aa) N-terminal extracellular domain (ECD) (aa 1-80), followed by a TM segment, a cytoplasmic region, a second TM segment and a C-terminal ECD (aa 142-152). Homophilic binding is divalent-cation dependent and occurs between Pro26 and Asn37 (2). Over aa 1-80, human Ninjurin-1 shares 84% aa sequence identity with mouse Ninjurin-1. Human Ninjurin-1 shares only 50% aa sequence identity with human Ninjurin-2. Expression of NINJ‑1 increased under inflammation conditions, and itself can also exert proinflammatory effects such as stimulating leukocyte migration, and activating TLR4 signaling pathway (3). Inhibiting hemophilic interaction of NINJ1 in circulating tumor cells greatly inhibit the mobility of the cancer cells (4).

References

  1. Araki, T. and Milbrandt J. (1996) Neuron 17:353.
  2. Araki, T. and Milbrandt J. (1997) J. Biol Chem 272:21373.
  3. Jennewein, C. et. al. (2015) Am J Respir Cell Mol Biol 53:656.
  4. Park, J. et. al. (2017) Anticancer Res 37:1687.

Alternate Names

NIN1, NINJ1, Ninjurin1

Entrez Gene IDs

4814 (Human); 18081 (Mouse); 25338 (Rat)

Gene Symbol

NINJ1

UniProt

Additional Ninjurin-1 Products

Product Documents for Recombinant Human Ninjurin-1 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Ninjurin-1 Protein, CF

For research use only

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