Recombinant Human Ninjurin-2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9719-NJ

R&D Systems, part of Bio-Techne
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9719-NJ-050
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Key Product Details

Source

E. coli

Accession #

Q9NZG7

Conjugate

Unconjugated

View all Ninjurin-2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Ninjurin-2 protein
Met1-Thr65, wtih a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1

Predicted Molecular Mass

8 kDa

SDS-PAGE

8 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9719-NJ
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ninjurin-2

Ninjurin-2 (nerve injury-induced protein 2) is a 20-22 kDa member of the Ninjurin family of transmembrane (TM) proteins (1). It is expressed by multiple cell types, including Schwann cells, myenteric plexus and sensory neurons, and lymphocytes and participates in intercellular homophilic binding (1). Human Ninjurin-2 is 142 amino acids (aa) in length. It has an unusual membrane orientation. There is a 65 aa N-terminal extracellular domain (ECD) (aa 1‑65) that contains one phoshorylation site at Ser3, followed by a TM segment, a cytoplasmic region, a second TM segment and a C-terminal ECD (aa 128‑142). One potential alternate start site exists 46 aa upstream of the standard form start site. Over aa 1‑65, human Ninjurin-2 is 71% aa identical to mouse Ninjurin-2. Multiple studies have linked NINJ-2 gene polymorphism with ischemic stroke (2-4). In endothelia cells, NINJ-2 activates TLR4 signaling pathway and promote inflammation (5).

References

  1. Araki, T. and J. Milbrandt (2000) J. Neurosci. 20:187.
  2. Zhu, Y. et al. (2014) Thromb Thrombolysis. 38:470.
  3. Bis. JC. et al. (2014) PLoS One 9:e99798.
  4. Li, BH., et al. (2012) J Neurol Sci 316:116.
  5. Wang, J. et al. (2017) Cell Signal 35:231.

Alternate Names

NINJ2, Ninjurin2

Entrez Gene IDs

4815 (Human); 29862 (Mouse); 59115 (Rat)

Gene Symbol

NINJ2

UniProt

Q9NZG7

Additional Ninjurin-2 Products

Product Documents for Recombinant Human Ninjurin-2 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Ninjurin-2 Protein, CF

For research use only

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