Recombinant Human NKG2C/CD159c Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 138-NK

R&D Systems, part of Bio-Techne
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138-NK-050
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Key Product Details

Source

HEK293

Accession #

P26717

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all NKG2C/CD159c Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human NKG2C/CD159c protein
MD
 Human IgG1
(Pro100-Lys330)
 IEGR
 Human NKG2C
(Glu98-Leu231)
Accession # P26717
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

42 kDa

SDS-PAGE

53-62 kDa, reducing conditions

Activity

Measured by its ability to bind Recombinant Human CD94 (Catalog # 9270-CD) in a functional ELISA. The ED50 for this effect is 0.3-2.4 µg/mL.

Scientific Data Images for Recombinant Human NKG2C/CD159c Fc Chimera Protein, CF

Recombinant Human NKG2C/CD159c Fc Chimera Protein Binding Activity

Recombinant Human NKG2C/CD159c Fc Chimera Protein Binding Activity

Recombinant Human NKG2C/CD159c Fc Chimera (Catalog # 138-NK) binds Recombinant Human CD94 (Catalog # 9270-CD) in a functional ELISA. The ED50 for this effect is 0.3-2.4 µg/mL.
Recombinant Human NKG2C/CD159c Fc Chimera Protein SDS-PAGE

Recombinant Human NKG2C/CD159c Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human NKG2C/CD159c (Catalog # 138-NK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 53-62 kDa and 110-120 kDa, respectively.

Formulation, Preparation and Storage

138-NK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKG2C/CD159c

Human NKG2C (NK cell Group 2 isoform C; Killer cell lectin-like receptor subfamily C, member 2) is a member of the C-type lectin-like superfamily of proteins. Natural killer (NK) receptors are expressed in both NK cells and cytotoxic CD8+ T cells and have both activating and inhibitory members (1-3). Regulation of the balance between the activating and inhibitory receptors is important and lack of such regulation has been implicated in autoimmunity (4). The NKG2 family includes seven receptors: NKG2A, -B, -C, -D, -E, -F, and -H, which is the longer isoform of NKG2E. Except for NKG2D and NKG2F, the NKG2 family members form heterodimers with CD94 (5, 6). NKG2C interacts with the adapter molecule DAP12 and acts as activating receptor when heterodimerized with CD94 (7). Human NKG2C is synthesized as a 231 amino acid (aa) protein that includes a 70 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 138 aa extracellular domain (ECD). Within the ECD, human NKG2C shares 40% sequence identity with mouse NKG2C. NKG2C-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (8, 9). Triggering the NKG2C-CD94 complex may activate the cytolytic activity and cytokine production of NK and CD8+ T cells (8, 10). Human cytomegalovirus (HCMV) infection promotes the differentiation and expansion of NKG2C+ NK cell subsets, possibly involving a cognate interaction of CD94/NKG2C with ligand(s) displayed by HCMV-infected cells (11, 12).

References

  1. Orbelyan, G.A. et al. (2014) J. Immunol. 193:610.
  2. Tassi I. et al. (2006) Immnunol Rev. 214:92.
  3. Lanier, L.L. (2008) Nat. Immunol. 9:495.
  4. Schleinitz, N. et al. (2010) Immunology. 174:2878.
  5. Lopez-Botet, M. et al. (2000) Hum. Immunol. 61:7.
  6. Braud, V.M. et al. (1998) Nature. 391:795.
  7. Lanier, L.L. (1998) Immunity 8:693.
  8. Vance, R.E. et al. (1999) J Exp Med 190:1801.
  9. Kaiser B.K. et al. (2005) J Immunol 174:2878.
  10. Bellón T. et al. (1999) J Immunol 162:3996.
  11. Pupuleku A. et al. (2017) Front Immunol. 8:1317.
  12. Hammer Q. et al. (2018) Nat Immunol. 19:453.

Long Name

Natural Killer G2C

Alternate Names

CD159c, KLRC2, NKG2-C

Entrez Gene IDs

3822 (Human); 16642 (Mouse)

Gene Symbol

KLRC2

UniProt

P26717

Additional NKG2C/CD159c Products

Product Documents for Recombinant Human NKG2C/CD159c Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human NKG2C/CD159c Fc Chimera Protein, CF

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