Recombinant Human NPDC-1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9724-NP

R&D Systems, part of Bio-Techne
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9724-NP-050
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Key Product Details

Source

NS0

Accession #

Q9NQX5

Conjugate

Unconjugated

View all NPDC-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human NPDC-1 protein
Gly35-Asp181, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly35

Predicted Molecular Mass

16 kDa

SDS-PAGE

25 - 39 Kda, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9724-NP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NPDC-1

Neural proliferation and differentiation control protein 1 (NPDC1) is an approximately 34 kDa protein that is mainly expressed in lung and neural tissues and whose expression is developmentally regulated and persists in the adult (1, 2). It binds a variety of cyclins and regulate differentiation events in neuronal precursor cells (3). NPDC1 also binds the transcription factor, E2F-1, which results in the inhibition of E2F-1 binding to DNA and the corresponding transcription events (4). The encoded protein of the human NPDC1 includes a 34 aa signal peptide, a 147 aa extracellular domain (ECD), a 21 aa transmembrane region, followed by a carboxyl terminus containing a PEST motif (rich in proline, glutamine, serine, and threonine) which targets the protein for degradation (1). Alignment of the mature protein shows that human NPDC1 shares 76% and 75% sequence identity with mouse and rat NPDC1, respectively.

References

  1. Spencer, M.L. et al. (2004) J. Biol. Chem. 279:37069.
  2. Evrard, C. and P. Rouget (2005) J. Neurosci. Res. 79:747.
  3. Galiana, E. et al. (1995) Proc. Natl. Acad. Sci. USA. 92:1560.
  4. Sansal, I. et al. (2000) Oncogene 19:5000.

Long Name

Neural Proliferation, Differentiation and Control, 1

Alternate Names

CAB1, NPDC1

Entrez Gene IDs

56654 (Human); 18146 (Mouse); 296562 (Rat)

Gene Symbol

NPDC1

UniProt

Q9NQX5

Additional NPDC-1 Products

Product Documents for Recombinant Human NPDC-1 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human NPDC-1 Protein, CF

For research use only

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