Recombinant Human NTB-A/SLAMF6 His tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9299-NT

R&D Systems, part of Bio-Techne
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9299-NT-050
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Key Product Details

Source

HEK293

Accession #

Q96DU3

Conjugate

Unconjugated

Applications

Bioactivity

View all NTB-A/SLAMF6 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human NTB-A/SLAMF6 protein
Gln22-Met226, with a C-teminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln22 inferred from enzymatic pyroglutamate treatment revealing Ser23

Predicted Molecular Mass

24 kDa

SDS-PAGE

34-42 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human NTB-A/SLAMF6 is coated at 1 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human
NTB-A/SLAMF6 Fc Chimera that produces 50% optimal binding response is 0.6-3 μg/mL.

Formulation, Preparation and Storage

9299-NT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NTB-A/SLAMF6

NTB-A, also known as Ly108 and SLAMF6, is a 60 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). Mature human NTB-A consists of a 205 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set and one Ig-like C2-set domain, a 21 aa transmembrane segment, and an 84 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs (ITSMs) (2, 3). An alternatively spliced isoform is truncated in the cytoplasmic domain and lacks the two ITSMs. Within the ECD, human NTB-A shares 48% aa sequence identity with mouse and rat NTB-A. The ECD of human NTB-A shares 19%-34% aa sequence identity with comparable regions of human 2B4, BLAME, CD2F-10, CD84, CD229, CRACC, and SLAM. NTB-A is expressed on the surface of NK, T, and B lymphocytes as well as eosinophils (2, 4, 5). It interacts homophilically through weak associations between the Ig-V domains (2, 5-7). NTB-A functions as an activating coreceptor on NK and T cells (2, 5, 6, 8). Tyrosine phosphorylation in the membrane proximal ITSM enables specific association with EAT-2, an interaction that is required for NTB-A mediated cytotoxicity of NK cells (9). Phosphorylation-dependent NTB-A association with SAP is required for full production of IFN-gamma by NK cells (5, 9). This interaction is independent of EAT-2 binding and appears to involve the membrane distal ITSM (5, 9). NTB-A deficient mice show weakened Th2 responses and elevated levels of neutrophil-derived inflammatory mediators (10). In B cells, NTB-A modulates immunoglobulin class switching and the balance between tolerance and autoimmunity (5, 11). In addition, NTB-A binds to the influenza virus hemagglutinin (HA) protein which co-stimulates NK cell activation (12). The Vpu protein encoded by HIV-1 interferes with NTB-A glycosylation and cell surface expression (13).

References

  1. Claus, M. et al. (2008) Front. Biosci. 13:956.
  2. Bottino, C. et al. (2001) J. Exp. Med. 194:235.
  3. Fraser, C.C. et al. (2002) Immunogenetics 53:843.
  4. Munitz, A. et al. (2005) J. Immunol. 174:110.
  5. Valdez, P.A. et al. (2004) J. Biol. Chem. 279:18662.
  6. Flaig, R.M. et al. (2004) J. Immunol. 172:6524.
  7. Cao, E. et al. (2006) Immunity 25:559.
  8. Stark, S. and C. Watzl (2006) Int. Immunol. 18:241.
  9. Eissmann, P. and C. Watzl (2006) J. Immunol. 177:3170.
  10. Howie, D. et al. (2005) J. Immunol. 174:5931.
  11. Kumar, K.R. et al. (2006) Science 312:1665.
  12. Duev-Cohen, A. et al. (2016) Oncotarget 7:13093.
  13. Bolduan, S. et al. (2013) Virology 440:190.

Long Name

NK-T-B Antigen/SLAM Family Member 6

Alternate Names

CD352, NTBA, SLAMF6

Entrez Gene IDs

114836 (Human)

Gene Symbol

SLAMF6

UniProt

Q96DU3

Additional NTB-A/SLAMF6 Products

Product Documents for Recombinant Human NTB-A/SLAMF6 His tag Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Human NTB-A/SLAMF6 His tag Protein, CF

For research use only

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