Recombinant Human P4HB Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4236-DI
Key Product Details
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human Protein Disulfide Isomerase/P4HB protein
Asp18-Lys505, with a C-terminal 10-His tag
Asp18-Lys505, with a C-terminal 10-His tag
Purity
>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Asp18
Predicted Molecular Mass
56 kDa
SDS-PAGE
60 kDa, reducing conditions
Activity
Measured by its ability to promote aggregation of insulin in the presence of DTT.
The specific activity is >7.5 A650/cm/min/mg, as measured under the described conditions.
The specific activity is >7.5 A650/cm/min/mg, as measured under the described conditions.
Formulation, Preparation and Storage
4236-DI
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Protein Disulfide Isomerase/P4HB
Protein Disulfide Isomerase, also known as prolyl 4‑hydroxylase subunit beta (P4HB), protocollagen hydroxylase, cellular thyroid hormone binding protein p55 and glutathione-insulin transhydrogenase (1-3) is an abundant multifunctional enzyme that belongs to the Protein Disulfide Isomerase family. It contains two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. When present as a tetramer consisting of two alpha subunits and two beta subunits, this enzyme functions as a hydroxylase catalyzing the hydroxylation of prolyl residues in preprocollagen. P4HB has various additional functions (4-7). It binds thyroid hormone. It acts as a chaperone that inhibits aggregation of misfolded proteins. It plays a role in both the influx and efflux of S-nitrosothiol-bound nitric oxide. It is also a subunit of the microsomal triglyceride transfer protein complex.
References
- Pihlajaniemi, T. et al. (1987) EMBO J. 6:643.
- Cheng, S.Y. et al. (1987) J. Biol. Chem. 262:11221.
- Morris, J. I. and Varandani, P. T. (1988) Biochim. Biophys. Acta 949:169.
- Obata, T. et al. (1988) J. Biol. Chem. 263:782.
- Gilbert, H. F. (1997) J. Biol. Chem. 272:29399.
- Sliskovic, I. et al. (2005) J. Biol. Chem. 280:8733.
- Wetterau, J. R. et al. (1990) J. Biol. Chem. 265:9800.
Long Name
Proline 4-Hydroxylase, Beta Polypeptide
Alternate Names
DSI, ERBA2L, GIT, PDI, PDIA1, PO4DB, PO4HB, PROHB, Thbp
Gene Symbol
P4HB
UniProt
Additional Protein Disulfide Isomerase/P4HB Products
Product Documents for Recombinant Human P4HB Protein, CF
Product Specific Notices for Recombinant Human P4HB Protein, CF
For research use only
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