Recombinant Human PGK-1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 5455-PK
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human PGK1 protein
Met1-Ile417, with an C-terminal 10-His tag
Met1-Ile417, with an C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No results obtained
Predicted Molecular Mass
46 kDa
SDS-PAGE
42 kDa, reducing conditions
Activity
Measured by NADH production in a reaction coupled with GAPDH.
The specific activity, as measured under the described conditions, is >50,000 pmol/min/μg.
The specific activity, as measured under the described conditions, is >50,000 pmol/min/μg.
Formulation, Preparation and Storage
5455-PK
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: PGK1
Phosphoglycerate kinase-1 (PGK-1) is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3 phosphoglycerate. The gene encoding PGK-1 is X-linked. Mutations of this gene may cause phosphoglycerate kinase deficiency, which is characterized by hemolytic anemia, muscle stiffness and mental retardation (1‑3). PGK 1 is induced by oxidative stress through the induction of hypoxia-inducible factor 1a and is a potential biomarker and therapeutic target for cancer (4‑7).
References
- Beutler, E. (2002) Br. J. Haematol. 136:3.
- Flanagan, J.M. et al. (2006) Br. J. Haematol. 134:233.
- Svaasand, E.K. et al. (2007) Muscle Nerve. 36:579.
- Hwang, T.L. et al. (2006) Proteomics. 6:2259.
- Wang, J. et al. (2007) Cancer Res. 67:149.
- Zieker, D. et al. (2008) Cell Physiol. Biochem. 21:429.
- Jang, C.H. et al. (2008) Biosci. Biotechnol. Biochem. 72:1799.
Long Name
Phosphoglycerate Kinase-1
Alternate Names
MIG10, PGKA, PRP2
Gene Symbol
PGK1
UniProt
Additional PGK1 Products
Product Documents for Recombinant Human PGK-1 Protein, CF
Product Specific Notices for Recombinant Human PGK-1 Protein, CF
For research use only
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