Recombinant Human PGLYRP4/PGRP-I beta Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8389-PG

R&D Systems, part of Bio-Techne
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8389-PG-050
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Key Product Details

Source

HEK293

Accession #

AAK72485

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all PGLYRP4/PGRP-I beta Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human PGLYRP4/PGRP-I beta protein
Asp18-His373 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp18

Predicted Molecular Mass

40 kDa

SDS-PAGE

53-64 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When peptidoglycan is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human PGLYRP4/PGRP-I beta that produces 50% optimal binding response is 0.5-3 μg/mL.

Formulation, Preparation and Storage

8389-PG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PGLYRP4/PGRP-I beta

PGLYRP4, also known as PGRP-I beta, is a member of the peptidoglycan recognition protein family of innate immunity proteins (1, 2). Human PGLYRP4 is expressed in the skin, eyes, salivary glands, throat, tongue, esophagus, stomach, and intestine (3). Mature human PGLYRP4 contains two nonidentical PGRP domains, and it shares 77% and 74% amino acid sequence identity with mouse and rat PGLYRP4, respectively (1, 4). It is secreted as disulfide-linked homodimers and binds peptidoglycan (PGN) and PGN-containing Gram-positive bacteria (1, 3). PGLYRP4 is directly bactericidal against pathogenic and nonpathogenic Gram-positive bacteria, but not normal flora bacteria, suggesting that normal flora bacteria have developed resistance to this bactericidal mechanism (3, 5, 6). Its bactericidal activity requires physiological concentrations of Zn2+ (6). PGLYRP4 knockout mice are more sensitive to the development of experimental dermatitis and DSS-induced colitis than wild type mice (2, 7). In humans, PGLYRP4 single nucleotide polymorphisms have been associated with inflammatory bowel disease and increased Parkinson’s disease risk (8, 9).

References

  1. Liu, C. et al. (2001) J. Biol. Chem. 276:34686.
  2. Park, S.Y. et al. (2011) PLoS One 6:e24961.
  3. Lu, X. et al. (2006) J. Biol. Chem. 281:5895.
  4. Royet, J. and R. Dziarski (2007) Nat. Rev. Microbiol. 5:264.
  5. Tydell, C.C. et al. (2006) J. Immunol. 176:1154.
  6. Wang, M. et al. (2007) J. Immunol. 178:3116.
  7. Saha, S. et al. (2010) Cell Host Microbe 8:147.
  8. Zulfiqar, F. et al. (2013) PLoS One 8:e67393.
  9. Goldman, S.M. et al. (2014) Mov. Disord. 29:1171.

Long Name

Peptidoglycan Recognition Protein Intermediate, Beta/Peptidoglycan Recognition Protein 4

Alternate Names

Pglyrp-1 beta, PGRP-I beta, PGRPI beta

Entrez Gene IDs

57115 (Human)

Gene Symbol

PGLYRP4

UniProt

AAK72485

Additional PGLYRP4/PGRP-I beta Products

Product Documents for Recombinant Human PGLYRP4/PGRP-I beta Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human PGLYRP4/PGRP-I beta Protein, CF

For research use only

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