Recombinant Human Phosphodiesterase 4A/PDE4A Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7767-PE

R&D Systems, part of Bio-Techne
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7767-PE-010
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

P27815

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Phosphodiesterase 4A/PDE4A Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Phosphodiesterase 4A/PDE4A protein
Pro331-Met723, with an N-terminal Met and a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro331

Predicted Molecular Mass

46 kDa

SDS-PAGE

44-48 kDa, reducing conditions

Activity

Measured by its ability to convert cAMP to 5'-AMP.
The specific activity is >28,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7767-PE
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Phosphodiesterase 4A/PDE4A

Cyclic nucleotide phosphodiesterases (PDEs) regulate the intracellular concentration of the second messengers, cAMP and cGMP (1-3). Phosphodiesterase 4, subtype A (PDE4A) is a rolipram-sensitive member of the cyclic phosphodiesterase family and catalyzes the specific hydrolysis of cAMP to 5'-AMP. Numerous inhibitors of PDE4 have been studied and considered as anti-inflammatory therapeutics for treatment of asthma, chronic obstructive pulmonary disease, rheumatoid arthritis, multiple sclerosis, type 2 diabetes, and potentially several CNS disorders (1-5). R&D Systems’ recombinant human PDE4A corresponds to the catalytic domain (6).

References

  1. Bender A.T. and J.A. Beavo (2006) Pharmacol. Rev. 58:488.
  2. Menniti F.S. et al. (2006) Nat. Rev. Drug Discov. 5:660.
  3. Omori K. and J. Kotera (2007) Circ. Res. 100:309.
  4. Spina D. (2008) Br. J. Pharmacol. 155:308.
  5. Wang, H. et al. (2007) Biochem. J. 408:193.
  6. Lario, P.I. et al. (2001) Arch. Biochem. Biophys. 394:54.

Alternate Names

DPDE2, PDE46, PDE4A

Entrez Gene IDs

5141 (Human); 18577 (Mouse); 25638 (Rat)

Gene Symbol

PDE4A

UniProt

P27815 (Human)

Additional Phosphodiesterase 4A/PDE4A Products

Product Documents for Recombinant Human Phosphodiesterase 4A/PDE4A Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Phosphodiesterase 4A/PDE4A Protein, CF

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