Recombinant Human Plexin C1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3887-PC

R&D Systems, part of Bio-Techne
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3887-PC-050
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Key Product Details

Source

NS0

Accession #

O60486

Conjugate

Unconjugated

Applications

Binding Activity

View all Plexin C1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Plexin C1 protein
Ala35-Thr944, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala35

Predicted Molecular Mass

100.9 kDa

SDS-PAGE

150-170 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Plexin C1 is coated at 5 μg/mL, Recombinant Human Semaphorin 7A Fc Chimera (Catalog # 2068-S7) binds with an apparent Kd <5 nM.

Reviewed Applications

Read 1 review rated 4 using 3887-PC in the following applications:

Formulation, Preparation and Storage

3887-PC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin C1

Plexin C1, also known as Virus-encoded semaphorin protein receptor (VESPR) and CD232, is a 210 kDa type I transmembrane glycoprotein in the C subfamily of the Plexin family (1, 2). Human Plexin C1 contains a 34 amino acid (aa) signal sequence, a 910 aa extracellular domain (ECD) with one Sema-domain and two cysteine-rich Met-related sequences (MRS), a 21 aa transmembrane domain and a 603 aa cytoplasmic domain that includes features common to other plexins, including a phosphothreonine site. The human Plexin C1 ECD shares 85%, 82% and 71% aa identity with murine, bovine and opossum Plexin C1, respectively. Plexin C1 is widely expressed in neuronal and non-neuronal fetal and adult tissues (3). In neuronal development, its role is unclear. Semaphorin-7a (Sema 7A, CD108) binds Plexin C1 in vitro, and the two show a similar expression pattern during rat neuronal development. However, in rat, beta1 integrins rather than Plexin C1 appear to mediate Sema 7A effects on axon outgrowth (4, 5). Plexin C1 does appear to play a role in the partitioning of paraventricular and supraoptic neurons in the hypothalamus, as indicated by specific defects seen in mice deleted for Plexin C1 (6). In the immune system, effects of Sema 7A on T cell-mediated inflammatory responses also appear to be mediated by beta1 integrins rather than plexins (7). However, Plexin C1 may function to oppose the effect of beta1 integrins, as it does on Sema 7A-mediated spreading and dendrite formation in melanocytes (8). Plexin C1 is the receptor for the poxvirus (A39R protein) and herpes virus (AHVsema) semaphorin homologs, and mediates activation of monocytes and inhibition of dendritic cell and neutrophil phagocytosis by A39R (2, 9, 10).

References

  1. Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363. 
  2. Comeau, M.R. et al. (1998) Immunity 8:473. 
  3. Perala, N.M. et al. (2005) Gene Expr. Patterns 5:355. 
  4. Pasterkamp, R.J. et al. (2007) BMC Dev. Biol. 7:98. 
  5. Pasterkamp, R.J. et al. (2003) Nature 424:398. 
  6. Xu, C. and C-M. Fan (2007) Mol. Endocrinol. 21:1234.
  7. Suzuki, K. et al. (2007) Nature 446:680.
  8. Scott, G.A. et al. (2007) J. Invest. Dermatol. 128:151.
  9. Walzer, T. et al. (2005) J. Immunol. 174:51.
  10. Walzer, T. et al. (2005) Eur. J. Immunol. 35:391.

Alternate Names

CD232, PLXNC1, VESPR

Entrez Gene IDs

10154 (Human); 54712 (Mouse); 362873 (Rat)

Gene Symbol

PLXNC1

UniProt

O60486

Additional Plexin C1 Products

Product Documents for Recombinant Human Plexin C1 Protein, CF

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Product Specific Notices for Recombinant Human Plexin C1 Protein, CF

For research use only

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