Recombinant Human PNP Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 6486-NP
Key Product Details
Product Specifications
Source
E. coli-derived human Purine Nucleoside Phosphorylase/PNP protein
Met1-Ser289, with a C-terminal 6-His tag
Met1-Ser289, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met1
Predicted Molecular Mass
33 kDa
SDS-PAGE
30-35 kDa, reducing conditions
Activity
Measured by the phosphorolysis of 7-methyl-6-thioguanosine.
The specific activity is >35,000 pmol/min/μg, as measured under the described conditions.
The specific activity is >35,000 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
6486-NP
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Purine Nucleoside Phosphorylase/PNP
References
- Schramm, V.L. (1998) Annu. Rev. Biochem. 67:693.
- Stoop, W. et al. (1977) N. Eng. J. Med. 296:651.
- Markert, M.L. (1991) Immunodefic. Rev. 3:45.
- Webb, M.R. (1992) Proc. Natl. Acad. Sci. USA. 89:4884.
Alternate Names
Inosine phosphorylase, PUNP
Entrez Gene IDs
4860 (Human)
Gene Symbol
PNP
UniProt
Additional Purine Nucleoside Phosphorylase/PNP Products
Product Documents for Recombinant Human PNP Protein, CF
Product Specific Notices for Recombinant Human PNP Protein, CF
Coomassie is a registered trademark of Imperial Chemical Industries Ltd.
For research use only
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