Recombinant Human PRCP Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7164-SE

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

P42785

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Lysosomal Pro-X Carboxypeptidase/PRCP Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Lysosomal Pro-X Carboxypeptidase/PRCP protein
Met1-His496, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu22

Predicted Molecular Mass

54 kDa

SDS-PAGE

60-70 kDa, reducing conditions

Activity

Measured by its ability to cleave the synthetic peptide substrate N-benzyloxycarbonyl-Pro-Ala (Z-Pro-Ala).
The specific activity is >2,500 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7164-SE
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Lysosomal Pro-X Carboxypeptidase/PRCP

Lysosomal Pro-X Carboxypeptidase (PRCP) is a serine carboxypeptidase found principally in lysosomes, but can also be secreted or associated with the plasma membrane. The enzyme is specific for peptide substrates with a penultimate proline residue. PRCP is also known as Angiotensinase C because of its activity against angiotensin II and angiotensin III (1). PRCP is also known to be involved in the activation of prekallikrein on the surface of endothelial cells (2). Because of these activities, PRCP is considered to be a cardioprotective enzyme (3). PRCP has been shown to degrade the bioactive peptide a-melonocyte stimulating hormone, thereby destroying its activity (4). Therefore PRCP may play a role in the melanocortin signaling pathway and the regulation of energy metabolism (5).

References

  1. Yang, H.Y.T. et al. (1968) Nature 218:1224.
  2. Shariat-Madar, Z. et al. (2002) J. Biol. Chem. 277:17962.
  3. Mallela, J. et al. (2009) Int. J. Biochem. Cell Biol. 41:477.
  4. Wallingford, N. et al. (2009) J. Clin. Invest. 119:2291.
  5. Diano, S. et al. (2011) Front. Neuroendocrinol. 32:70.

Alternate Names

Angiotensinase C, HUMPCP, Prolylcarboxypeptidase

Entrez Gene IDs

5547 (Human); 72461 (Mouse); 293118 (Rat)

Gene Symbol

PRCP

UniProt

P42785

Additional Lysosomal Pro-X Carboxypeptidase/PRCP Products

Product Documents for Recombinant Human PRCP Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human PRCP Protein, CF

For research use only

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