Recombinant Human PRDC/GREM2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8436-PR

R&D Systems, part of Bio-Techne
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8436-PR-050
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Key Product Details

Source

E. coli

Accession #

Q9H772

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all PRDC/GREM2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human PRDC/GREM2 protein
Arg22-Gln168 with an N-terminal Met

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

17 kDa

SDS-PAGE

18 kDa, reducing conditions

Activity

Measured by its ability to inhibit BMP-4-induced activity in MC3T3-E1 mouse preosteoblast cells.
The ED50 for this effect is 0.03-0.12 μg/mL

Scientific Data Images for Recombinant Human PRDC/GREM2 Protein, CF

Recombinant Human PRDC/GREM2 Protein Bioactivity

Recombinant Human PRDC/GREM2 Protein Bioactivity

Recombinant Human PRDC/GREM2 (Catalog # 8436-PR) inhibits BMP-4-induced activity in MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 0.03-0.12 μg/mL

Formulation, Preparation and Storage

8436-PR
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution
Reconstitute at 500 μg/mL in 4 mM HCl

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PRDC/GREM2

PRDC/GREM2 (Protein Related to DAN and Cerberus/Gremlin-2) is a secreted BMP antagonist belonging to the Cerberus/DAN (CAN) family. Similar to other CAN family members, PRDC/GREM2 has 6 conserved cysteine residues that form a cysteine knot, and two additional cysteine residues located in the loops of the cysteine knot (1-3). Mature human PRDC/GREM2 is synthesized as a 168 amino acid (aa) monomer. At the amino acid level, mature human PRDC/GREM2 shares 97% sequence identity with mature mouse PRDC/GREM2. PRDC/GREM2 is biologically active as a homodimer that assembles its subunits in a head-to-tail orientation. PRDC/GREM2 is up-regulated through Wnt/ beta-catenin signaling and has been shown to potently inhibit BMP-2, -4, and -7 (4-6). During early development PRDC/GREM2 is involved in tooth morphogenesis, osteogenesis, and neurogenesis (7-9). In the adult, PRDC/GREM2 is involved in bone repair and bone remodeling (10). PRDC/GREM2 has been detected in the adult ovary, brain, spleen, and colon (11). The biological activity of PRDC/GREM2 can be blocked by binding to heparin (4).

References

  1. Pearce, J.J. et al. (1999) Dev. Biol. 209:98.
  2. Kattamuri, C. et al. (2012) Protein Expr. Purif. 82:389.
  3. Minabe-Saegusa, C. et al. (1998) Dev. Growth Differ. 40:343.
  4. Nolan, K. et al. (2013) Structure 21:1417.
  5. Kattamuri, C. et al. (2012) J. Mol. Biol. 424:313.
  6. Im, J. et al. (2007) Biochem. Biophys. Res. Commun. 354:296.
  7. Vogel, P. et al. (2014) Vet. Pathol. [Epub ahead of print; PMID 24686385].
  8. Kriebitz, N.N. et al. (2009) Dev. Biol. 336:280.
  9. Ideno, H. et al. (2009) Exp. Cell Res. 315:474.
  10. Dean, D.B. et al. (2010) J. Anat. 216:625.
  11. Sudo, S. et al. (2004) J. Biol. Chem. 279:23134.

Long Name

Protein Related to DAN and Cerberus/Gremlin-2

Alternate Names

CKTSF1B2, DAND3, GREM2

Entrez Gene IDs

64388 (Human); 23893 (Mouse)

Gene Symbol

GREM2

UniProt

Q9H772

Additional PRDC/GREM2 Products

Product Documents for Recombinant Human PRDC/GREM2 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human PRDC/GREM2 Protein, CF

For research use only

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