Recombinant Human PRL-1/PTP4A1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8490-PT

R&D Systems, part of Bio-Techne
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8490-PT-050
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Key Product Details

Source

E. coli

Accession #

Q93096

Conjugate

Unconjugated

Applications

Enzyme Activity

View all PRL-1/PTP4A1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human PRL-1/PTP4A1 protein
Ala2-Gln173, with an N-terminal Met and 7-His tag

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

21 kDa

SDS-PAGE

24 kDa, reducing conditions

Activity

Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP).
The specific activity is >0.35 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8490-PT
Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, Betamercaptoethanol and Brij-35.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: PRL-1/PTP4A1

Phosphatase of regenerating liver 1 (PRL-1), also known as protein-tyrosine phosphatase 4A1 (PTP4A1), is a member of the PRL subgroup of PTPases (1). It was originally identified as an immediate-early gene in regenerating liver and later shown to be a PTPase that modulates cell growth (2, 3). PRL-1 is widely expressed in human tissues, and the rat enzyme is expressed in neurons and oligodendrocytes of the cerebral cortex, hippocampus, and cerebellum (4, 5). Human PRL-1 shares 100% amino acid sequence identity with mouse and rat PRL-1. The crystal structure of PRL-1 shows that the protein exists as a trimer (6, 7). PRL-1 localizes to the endoplasmic reticulum in interphase cells in a farnesylation-dependent manner, and to centrosomes during mitosis in a farnesylation-independent manner (8, 9). PRL-1 has been implicated in cell migration, invasion, and metastasis, suggesting that it may play a role in cancer (10-13). PRL-1 promotes colorectal cancer cell growth, migration, and invasion in vitro and tumor growth in vivo (14). Additionally, elevated PRL-1 expression is correlated with tumor progression in human hepatocellular carcinoma (15).

References

  1. Bessette, D.C. et al. (2008) Cancer Metastasis Rev. 27:231.
  2. Mohn, K.L. et al. (1991) Mol. Cell. Biol. 11:381.
  3. Diamond, R.H. et al. (1994) Mol. Cell. Biol. 14:3752.
  4. Dumaual, C.M. et al. (2006) J. Histochem. Cytochem. 54:1401.
  5. Takano, S. et al. (1996) Brain Res. Mol. Brain Res. 40:105.
  6. Sun, J.P. et al. (2005) Biochemistry 44:12009.
  7. Jeong, D.G. et al. (2005) J. Mol. Biol. 345:401.
  8. Zeng, Q. et al. (2000) J. Biol. Chem. 275:21444.
  9. Wang, J. et al. (2002) J. Biol. Chem. 277:46659.
  10. Zeng, Q. et al. (2003) Cancer Res. 63:2716.
  11. Achiwa, H. and J.S. Lazo (2007) Cancer Res. 67:643.
  12. Nakashima, M. and J.S. Lazo (2010) J. Pharmacol. Exp. Ther. 334:627.
  13. Jin, S. et al. (2014) Oncotarget 5:3685.
  14. Zhou, C. et al. (2013) PLoS One 8:e63142.
  15. Lu, J.W. et al. (2012) Clin. Transl. Oncol. 14:287.

Long Name

Phosphatase of Regenerating Liver 1

Alternate Names

HH72, PRL1, PTP(CAAX1)

Entrez Gene IDs

7803 (Human); 19243 (Mouse); 102547074 (Rat)

Gene Symbol

PTP4A1

UniProt

Q93096

Additional PRL-1/PTP4A1 Products

Product Documents for Recombinant Human PRL-1/PTP4A1 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human PRL-1/PTP4A1 Protein, CF

For research use only

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