Recombinant Human PRL-2/PTP4A2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 6694-PT
Key Product Details
Product Specifications
Source
E. coli-derived human PRL-2/PTP4A2 protein
Asn2-Gln167, with an N-terminal Met and 6-His tag
Asn2-Gln167, with an N-terminal Met and 6-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
20 kDa
SDS-PAGE
20-22 kDa, reducing conditions
Activity
Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP).
The specific activity is >0.3 pmol/min/μg, as measured under the described conditions.
The specific activity is >0.3 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
6694-PT
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl and Betamercaptoethanol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: PRL-2/PTP4A2
Phosphatase of regenerating liver 2 (PRL-2), also known as protein tyrosine phosphatase 4A2 (PTP4A2) or OV-1, dephosphorylates tyrosine residues. PRL-2, similar to PRL-1 and PRL-3, is an oncogenic protein and considered a candidate cancer biomarker and therapeutic target (1, 2, 3). Over‑expression of PRL-2 correlates with tumor formation and progression, and overall survival depends on estrogen and progestin receptor status (2, 4). In lung cancer cells, PRL-2 promotes cell migration and invasion through an ERK-dependent signaling pathway (5), suppresses p21 promoter activity, and promotes AP-1 activity (6). PRL-2 also is essential for placental development by down-regulating PTEN and activating Akt Protein (7).
References
- Stephens, B.J. et al. (2005) Mol. Cancer Ther. 4:1653.
- Andres, S.A. et al. (2013) Horm. Cancer 4:2083.
- Rios,P. et al. (2013) FEBS J. 280:505.
- Hardy, S. et al. (2010) Cancer Res. 70:8959.
- Wang, Y. and J.S. Lazo. (2012) Oncogene 31:818.
- Hwang, J.J. et al. (2012) Oncol. Rep. 27:535.
- Dong, Y. et al. (2012) J. Biol. Chem. 287:32172.
Long Name
Phosphatase of Regenerating Liver 2
Alternate Names
HH13, HH7-2, HU-PP-1, OV-1, PRL2, PTP4A2, PTPCAAX2
Entrez Gene IDs
8073 (Human)
Gene Symbol
PTP4A2
UniProt
Additional PRL-2/PTP4A2 Products
Product Documents for Recombinant Human PRL-2/PTP4A2 Protein, CF
Product Specific Notices for Recombinant Human PRL-2/PTP4A2 Protein, CF
For research use only
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