Recombinant Human Pro-Collagen II Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7589-CL

R&D Systems, part of Bio-Techne
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7589-CL-020
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Key Product Details

Source

CHO

Accession #

P02458

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Collagen II Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Collagen II protein
Gln26-Arg266 & Gly957-Leu1487, with an N-terminal 5-His tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His & Asp1242

Predicted Molecular Mass

78 kDa

SDS-PAGE

92-100 kDa, 65-70 kDa, 36 kDa, reducing conditions

Activity

Measured by the cleavage of its C-terminal propeptide by Recombinant Human BMP-1/PCP (Catalog # 1927-ZN).
>50% of full-length Pro-Collagen II is cleaved by Recombinant Human BMP‑1/PCP (Catalog # 1927-ZN), as measured under the described conditions.

Formulation, Preparation and Storage

7589-CL
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Collagen II

Type II collagen is an essential component of cartilage. It is essential for skeletal development and for the ability of cartilage to resist compressive forces. Type II collagen is a homotrimer of a1(II) chains. Defects in Type II collagen are the cause of numerous diseases, including chondrodysplasias and arthritis (1). This recombinant mini pro-a1(II) collagen consists of the N‑terminal propeptide, 108 Gly-X-Y repeats, and the C‑terminal propeptide. Proteolytic processing of the C‑terminal propeptide by the procollagen C-proteinase (BMP-1) releases chondrocalcin, a calcium binding protein.

References

  1. Kuivaniemi H. et al. (1997) Hum. Mutat. 9:300.

Alternate Names

Chondrocalcin, COL2A1, SEDC

Entrez Gene IDs

1280 (Human); 12824 (Mouse); 25412 (Rat)

Gene Symbol

COL2A1

UniProt

P02458 (Human)

Additional Collagen II Products

Product Documents for Recombinant Human Pro-Collagen II Protein, CF

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Product Specific Notices for Recombinant Human Pro-Collagen II Protein, CF

For research use only

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