Recombinant Human Pro-Collagen III alpha 1/COL3A1, CF
R&D Systems, part of Bio-Techne | Catalog # 7294-CL
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Collagen III alpha 1/COL3A1 protein
Thr1097-Leu1466, with an N-terminal 6-His tag
Thr1097-Leu1466, with an N-terminal 6-His tag
Purity
>75%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
His
Predicted Molecular Mass
40 kDa
SDS-PAGE
41-46 kDa, reducing conditions
Activity
Measured by the cleavage of its C-terminal propeptide by Recombinant Human BMP-1/PCP (Catalog # 1927-ZN).
>50% of full-length Pro-Collagen III alpha1 is cleaved by recombinant human BMP-1, as measured under the described conditions.
>50% of full-length Pro-Collagen III alpha1 is cleaved by recombinant human BMP-1, as measured under the described conditions.
Formulation, Preparation and Storage
7294-CL
| Formulation | Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Collagen III alpha 1/COL3A1
alpha1(III) chains that are linked by interchain disulfide bonds. Defective collagen type III is a cause of Ehlers-Danlos syndrome types III and IV and arterial aneurisms (1). This recombinant mini pro-alpha 1(III) collagen consists of a triple-helical region of Gly-Xaa-Yaa repeats, a short non-helical region, and the C‑terminal propeptide. The C‑terminal propeptide can be removed by the procollagen C‑proteinase (BMP-1).
References
- Kuivaniemi H. et al. (1997) Hum. Mutat. 9:300.
Alternate Names
COL3A1, EDS4A
Gene Symbol
COL3A1
UniProt
Additional Collagen III alpha 1/COL3A1 Products
Product Documents for Recombinant Human Pro-Collagen III alpha 1/COL3A1, CF
Product Specific Notices for Recombinant Human Pro-Collagen III alpha 1/COL3A1, CF
Coomassie is a registered trademark of Imperial Chemical Industries Ltd.
For research use only
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