Recombinant Human Proprotein Convertase 7/PCSK7 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2984-SE
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Proprotein Convertase 7/PCSK7 protein
Leu38-Thr667, with a 10-His tag between Arg141 and Ser142
Leu38-Thr667, with a 10-His tag between Arg141 and Ser142
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Leu38 & His tag
Predicted Molecular Mass
70 kDa (pro form) & 59 kDa (mature form)
SDS-PAGE
79 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate pERTKR-AMC (Catalog # ES013).
The specific activity is >125 pmol/min/µg, as measured under the described conditions.
The specific activity is >125 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2984-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Proprotein Convertase 7/PCSK7
The human PCSK7 gene encodes proprotein convertase 1 (PC7), which is also known as PC8 and lymphoma PC (1, 2). As a serine protease of the furin/PC family, the deduced amino acid sequence of human PC7 consists of a signal peptide (residues 1 to 37), a propeptide (residue 38 to 141), and a mature chain (residues 142 to 785) that consists of extracellular (residues 142 to 667), transmembrane (residues 668 to 688) and cytoplasmic (residues 689 to 785) domains. Autocatalytic processing results in the removal of the propeptide, which confers potent inhibitory activity toward the enzyme (3-5). The purified recombinant human PCSK7 corresponds to the extracellular domain of the mature chain with a 10X His tag at the N-terminus. However, it may also contain the propeptide, as suggested by the N-terminal sequencing results. Thermolysin treatment increases its activity, which may be due to degradation of the propeptide by thermolysin.
References
- Bruzzaniti, A. et al. (1996) Biochem. J. 314:727.
- Meerabux, J. et al. (1996) Cancer Res. 56:448.
- Van de Loo, J.-W.H.P. et al. (1997) J. Biol. Chem. 272:27116.
- Bhattacharjya, S. et al. (2000) Biochemistry 39:2868.
- Seidah, N.G. and M. Chretien (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 1877, Academic Press, San Diego.
Alternate Names
LPC, PCSK7, SPC7
Gene Symbol
PCSK7
UniProt
Additional Proprotein Convertase 7/PCSK7 Products
Product Documents for Recombinant Human Proprotein Convertase 7/PCSK7 Protein, CF
Product Specific Notices for Recombinant Human Proprotein Convertase 7/PCSK7 Protein, CF
For research use only
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