Recombinant Human Prostasin/Prss8 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4599-SE
Key Product Details
Source
CHO
Accession #
Structure / Form
Active
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human Prostasin/Prss8 protein
Ala33-Gly319, with a C-terminal 10-His tag
The pro form was activated and further purified.
Ala33-Gly319, with a C-terminal 10-His tag
The pro form was activated and further purified.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala33 & Ile45
Predicted Molecular Mass
32 kDa (Pro) & 31 kDa (Mature)
SDS-PAGE
39 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC (Catalog # ES014).
The specific activity is >10 pmol/min/µg, as measured under the described conditions.
The specific activity is >10 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
4599-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Prostasin/Prss8
Prostasin, also known as channel activating protease 1, is a serine protease with trypsin-like substrate specificity (1, 2). The preproenzyme possesses a C-terminal membrane-spanning domain that can be proteolytically processed to generate a secreted form of the enzyme. The secreted form of Prostasin has been purified from seminal fluid (2). Prostasin is highly expressed in the prostate gland, and is expressed at lower levels in the lung, kidney, liver, salivary gland, and pancreas (3). The peptidase activity of Prostasin is involved in the regulation of epithelial sodium channels (4, 5).
References
- Vuagniaux, G. et al. (2000) J. Am. Soc. Nephrol. 11:828.
- Yu, J.X. et al. (1994) J. Biol. Chem. 269:18843.
- Yu, J.X. et al. (1995) J. Biol. Chem. 270:13483.
- Caldwell, R.A. et al. (2004) Am. J. Physiol. Cell Physiol. 286:C194.
- Tong, Z. et al. (2004) Am. J. Physiol. Lung Cell Mol. Physiol. 287:L928.
Alternate Names
Prss8
Gene Symbol
PRSS8
UniProt
Additional Prostasin/Prss8 Products
Product Documents for Recombinant Human Prostasin/Prss8 Protein, CF
Product Specific Notices for Recombinant Human Prostasin/Prss8 Protein, CF
For research use only
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