Recombinant Human Protocadherin-12 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8477-CA

R&D Systems, part of Bio-Techne
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8477-CA-050
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Key Product Details

Source

CHO

Accession #

AAH52973

Conjugate

Unconjugated

Applications

Bioactivity

View all Protocadherin-12 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Protocadherin-12 protein
Gln25-Ser705, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained.Gln25 inferred from enzymatic pyroglutamate treatment revealing Glu26

Predicted Molecular Mass

75 kDa

SDS-PAGE

80-100 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells.
The ED50 for this effect is 0.2-1.0 μg/mL.

Formulation, Preparation and Storage

8477-CA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protocadherin-12

Protocadherin-12 (PCDH12; also called VE-Cadherin-2) is an approximately 150 kDa glycoprotein belonging to the delta2 subgroup of non-clustered protocadherins. These are type I transmembrane proteins that exhibit calcium-dependent but relatively weak homophilic adhesion (1-4). Like other delta2 protocadherins, mature Protocadherin-12 contains six cadherin domains in its extracellular domain (ECD), a transmembrane sequence, and a cytoplasmic domain (1). Within the ECD, human Protocadherin-12 shares 83% and 82% amino acid sequence identity with mouse and rat Protocadherin-12, respectively. Protocadherin-12 is expressed in mouse endothelial,
glycogen-rich trophoblast, and mesangial cells where it clusters at intercellular junctions (1, 2). It may be important during placental development, as placentas from Protocadherin-12-deficient mice display altered morphogenesis and transcription profiles (3). In humans, the ECD of Protocadherin-12 can be shed via proteolytic cleavage (5). It circulates at increased levels in sera from pregnant women exhibiting pre-eclampsia compared to women with nonpathological gestations (5).rich

References

  1. Telo’, P. et al. (1998) J. Biol. Chem. 273:17565.
  2. Rampon, C. et al. (2005) Exp. Cell Res. 302:48.
  3. Rampon, C. et al. (2008) Physiol. Genomics 34:193.
  4. Kim, S.Y. et al. (2011) Cell Adh. Migr. 5:97.
  5. Bouillot, S. et al. (2011) J. Biol. Chem. 286:15195.

Alternate Names

PCDH12, Protocadherin12, VE-cad-2, VE-cadherin-2

Entrez Gene IDs

51294 (Human); 53601 (Mouse)

Gene Symbol

PCDH12

UniProt

AAH52973

Additional Protocadherin-12 Products

Product Documents for Recombinant Human Protocadherin-12 Protein, CF

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Product Specific Notices for Recombinant Human Protocadherin-12 Protein, CF

For research use only

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