Recombinant Human Protocadherin-18 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8315-CA

R&D Systems, part of Bio-Techne
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8315-CA-050
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Key Product Details

Source

CHO

Accession #

Q9HCL0

Conjugate

Unconjugated

Applications

Bioactivity

View all Protocadherin-18 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Protocadherin-18 protein
Lys28-Ser699, with a C-terminal 6-­His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys28

Predicted Molecular Mass

75 kDa

SDS-PAGE

90-105 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of BCE C/D-1b bovine corneal endothelial cells.
The ED50 for this effect is 0.04-0.2 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

8315-CA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 400 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protocadherin-18

Protocadherin-18 (PCDH18) is an approximately 130 kDa glycoprotein belonging to the delta2 subgroup of non-clustered protocadherins (1-3). Like other delta2 protocadherins, mature Protocadherin-12 contains six cadherin domains in its extracellular domain (ECD), a transmembrane sequence, and a cytoplasmic domain (1, 2, 4). Within the ECD, human Protocadherin-18 shares 92% and 91% amino acid sequence identity with mouse and rat Protocadherin-18, respectively. Protocadherin-18 is expressed in the brain, heart, kidney, lung, and trachea (1, 5). Protocadherin-18 promotes cell-cell adhesion during embryogenesis in Zebrafish (6). Mouse Protocadherin-18 interacts with Disabled-1, a protein required for correct formation of cortical neuron layers (4, 5, 7, 8). Also in mice, Protocadherin-18 may additionally be an activation marker for CD8+ memorty T cells (9). In humans, deletion of Protocadherin-18 has been linked to intellectual disability (10).

References

  1. Wolverton, T. and M. Lalande (2001) Genomics 76:66.
  2. Vanhalst, K. et al. (2005) Cell. Mol. Life Sci. 62:1247.
  3. Kim, S.Y. et al. (2011) Cell Adh. Migr. 5:97.
  4. Redies, C. et al. (2005) Cell. Mol. Life Sci. 62:2840.
  5. Homayouni, R. et al. (2001) Biochem. Biophys. Res. Commun. 289:539.
  6. Aamar, E. and I.B. Dawid (2008) Dev. Biol. 318:335.
  7. Howell, B.W. et al. (1999) Genes Dev. 13:643.
  8. Howell, B.W. et al. (2000) Curr. Biol. 10:877.
  9. Vazquez-Cintron, E.J. et al. (2012) PLoS One 7:e36101.
  10. Kasnauskiene, J. et al. (2012) Eur. J. Med. Genet. 55:274.

Alternate Names

KIAA1562, PCDH68L1, Protocadherin18

Entrez Gene IDs

54510 (Human); 73173 (Mouse); 295027 (Rat)

Gene Symbol

PCDH18

UniProt

Q9HCL0

Additional Protocadherin-18 Products

Product Documents for Recombinant Human Protocadherin-18 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Protocadherin-18 Protein, CF

For research use only

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