Recombinant Human PTPRD Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9995-PR

R&D Systems, part of Bio-Techne
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9995-PR-050
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Key Product Details

Source

HEK293

Accession #

P23468

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all PTPRD Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human PTPRD protein
Human PTPRD
(Glu21-Ser1174)
Accession # P23468		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu21

Predicted Molecular Mass

154 kDa

SDS-PAGE

137-151 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human PTPRD Fc Chimer is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human IL-1 RAPL1/IL-1 R8 Fc Chimera (Catalog # 9949-ML) binds with an ED50 of 1.5-9 ng/mL. 

Scientific Data Images for Recombinant Human PTPRD Fc Chimera Protein, CF

Recombinant Human PTPRD Fc Chimera Protein Binding Activity

Recombinant Human PTPRD Fc Chimera Protein Binding Activity

When Recombinant Human PTPRD Fc Chimera is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human IL-1 RAPL1/IL-1 R8 Fc Chimera (Catalog # 9949-ML) binds with an ED50 of 1.5-9 ng/mL.

Formulation, Preparation and Storage

9995-PR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: PTPRD

Human protein-tyrosine phosphatase delta (PTPRD) is a type I membrane protein. It contains a 1245 amino acid (aa) long extracellular domain (ECD), a 25 amino acid long transmembrane domain, and a 622 amino acid cytoplasmic domain. A cleavage during post-translational modification separates the extracellular domain from the transmembrane segment through a process called "ectodomain shedding" (1). The extracellular regions are comprised of three Ig-like C2 domains followed immediately by eight fibronectin type-III like domains (1). The human PTPRD extracellular domain shares 98% and 62% aa identity with mouse and rat PTPRD, respectively. Protein-tyrosine phosphatases (PTPs) constitute a structurally diverse family of tightly regulated enzymes with important regulatory roles (1-6). PTPRD is a member of the PTPs and is detected in brain and other tissues including colon and breast (1). It has been demonstrated that phosphorylated STAT3 (p-STAT3) is a direct substrate of PTPRD and that cancer-specific mutations in PTPRD abrogate its ability to dephosphorylate STAT3 (5). PTPRD interacts with NGL-3 (Netrin-G ligand-3) via its first two FNIII repeats to promote synapse formation (3). PTPRD can also bind to IL1RAPL1 and its paralog IL1RAPL2; the IL1RAPL1/PTPRD complex recruits RhoGAP2 at excitatory synapses to induce dendritic spine formation (4). Recent studies have indicated SALM5 forms heterotetramer with PTPRD to induce synaptic differentiation (6).

References

  1. Pulido, R. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11686.
  2. Östman, A. et al. (2006) Nature Reviews Cancer 6:307.
  3. Kwon, SK. et al. (2010) J. Biol. Chem. 285:13966.
  4. Valnegri, P. et al. (2011) Hum. Mol. Genet. 20:4797.
  5. Ortiz, B. et al. (2014) Proc. Natl. Acad. Sci. USA. 111:8149.
  6. Lin, Z. et al. (2018) Nat, Commun. 9:268.

Long Name

Protein Tyrosine Phosphatase Receptor-type delta

Alternate Names

PTP delta, PTP-delta, PTPD, PTPdelta, R-PTP-Delta, RPTP-Delta

Entrez Gene IDs

5789 (Human); 19266 (Mouse); 313278 (Rat)

Gene Symbol

PTPRD

UniProt

P23468

Additional PTPRD Products

Product Documents for Recombinant Human PTPRD Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human PTPRD Fc Chimera Protein, CF

For research use only

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