Recombinant Human QSOX1/Quiescin Q6 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9209-QS

R&D Systems, part of Bio-Techne
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9209-QS-020
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Key Product Details

Source

E. coli

Accession #

O00391

Conjugate

Unconjugated

Applications

Enzyme Activity

View all QSOX1/Quiescin Q6 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human QSOX1/Quiescin Q6 protein
Ser33-Ala546, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

58 kDa

SDS-PAGE

55 kDa, reducing conditions

Activity

Measured by its ability to produce hydrogen peroxide during the oxidation of Dithiothreitol (DTT).
The specific activity is >1,200 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

9209-QS
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: QSOX1/Quiescin Q6

Sulfhydryl Oxidase-1 (QSOX1) is an approximately 80 kDa enzyme that contains thioredoxin and sulfhydryl oxidase domains (1-3). It is synthesized with a C-terminal transmembrane segment, but soluble secreted forms can be generated by alternative splicing or proteolytic shedding within the Golgi (4). Within the region encompassing both enzymatic domains and the central region, human QSOX1 shares 79% aa sequence identity with mouse and rat QSOX1. It plays a role nascent protein folding by mediating disulfide oxidation (4-6). This activity is required for Laminin incorporation into the extracellular matrix (7). QSOX1 is up-regulated in many cancers and supports tumor cell proliferation and invasion (1, 8).

References

  1. Lake, D.F. and D.O. Faigel (2014) Antioxid. Redox. Signal. 21:485.
  2. Kodali, V.K. and C. Thorpe (2010) Antioxid. Redox Signal. 13:1217.
  3. Coppock, D.L. et al. (1998) Genomics 54:460.
  4. Rudolf, J. et al. (2013) Biochem. J. 454:181.
  5. Heckler, E.J. et al. (2008) Biochemistry 47:4955.
  6. Alon, A. et al. (2012) Nature 488:414.
  7. Ilani, T. et al. (2013) Science 341:74.
  8. Katchman, B.A. et al. (2011) Mol. Cancer Res. 9:1621.

Long Name

Quiescin Q6 Sulfhydryl Oxidase 1

Alternate Names

HQSOX, QSCN6, Quiescin Q6, Sulfhydryl Oxidase-1

Entrez Gene IDs

5768 (Human); 104009 (Mouse); 84491 (Rat)

Gene Symbol

QSOX1

UniProt

O00391

Additional QSOX1/Quiescin Q6 Products

Product Documents for Recombinant Human QSOX1/Quiescin Q6 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human QSOX1/Quiescin Q6 Protein, CF

For research use only

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