Recombinant Human RECK Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 10309-RE
Key Product Details
Source
HEK293
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Human embryonic kidney cell, HEK293-derived human RECK protein
Human RECK (Met1-Pro941) Accession # O95980 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly27
Predicted Molecular Mass
127 kDa
SDS-PAGE
125-137 kDa, under reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Human RECK Fc Chimera (Catalog # 10309-RE) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human TEM5/GPR124 His-tag (Catalog # 10206-TE) binds with an ED50 of 0.7-5.6 μg/mL.
When Recombinant Human RECK Fc Chimera (Catalog # 10309-RE) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human TEM5/GPR124 His-tag (Catalog # 10206-TE) binds with an ED50 of 0.7-5.6 μg/mL.
Scientific Data Images for Recombinant Human RECK Fc Chimera Protein, CF
Recombinant Human RECK Fc Chimera Protein Binding Activity
When Recombinant Human RECK Fc Chimera (Catalog # 10309-RE) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human TEM5/GPR124 His-tag (Catalog # 10206-TE) binds with an ED50 of 0.7-5.6 µg/mL.Recombinant Human RECK Fc Chimera Protein SDS-PAGE
2 μg/lane of Recombinant Human RECK Fc Chimera (Catalog # 10309-RE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band under reducing conditions at ~131 kDa.Formulation, Preparation and Storage
10309-RE
Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. |
Reconstitution | Reconstitute at 500 μg/mL in water. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: RECK
References
- Alexius-Lindgren, M. et al. (2014) Anticancer Res. 34:3867.
- Takahashi, C. et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95:13221.
- Clark, J.C.M. et al. (2007) Cancer Mestast. Rev. 26:675.
- Vallon, M. et al. (2018) Cell Reports. 25:339.
- Cho, C. et al. (2019) Elife. 8:e47300.
- Oh, J. et al. (2001) Cell. 107:789.
- Takeuchi, T. et al. (2004) Clin. Cancer Res. 10:5572.
- Zhang, G. et al. (2012) Cancer Sci. 103:1084.
- Masui, T. et al. (2003) Clin. Cancer Res. 9:1779.
- Chung, T.T. et al. (2012) PLoS ONE. 7:e33517.
- Miki, T. et al. (2007) J. Biol. Chem. 282:12341.
- Matsuzaki, T. et al. (2018) Biol. Open 7:bio033985.
- Muraguchi, T. et al. (2007) Nat. Neurosci. 10:838.
- Liu, Y. et al. (2018) J. Cell. Biochem. 119:3058.
Long Name
Reversion-inducing-cysteine-rich Protein with Kazal Motifs
Alternate Names
ST15
Gene Symbol
RECK
UniProt
Additional RECK Products
Product Documents for Recombinant Human RECK Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human RECK Fc Chimera Protein, CF
For research use only
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