Recombinant Human ROBO3 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3076-RB
Key Product Details
Source
Accession #
Structure / Form
Conjugate
Applications
Product Specifications
Source
Human ROBO3 (Leu40-Ser545) Accession # Q96MS0 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
N-terminus | C-terminus |
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Biotinylated recombinant mouse Slit-3 captured on a streptavidin-coated plate at 1 µg/mL can bind Recombinant Human ROBO3 Fc Chimera with an apparent Kd <10 nM.
Formulation, Preparation and Storage
3076-RB
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
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Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: ROBO3
Human ROBO3 is a 200 kDa member of the ROBO family of guidance molecules (1-3). The term ROBO derives from round-about, a description of the circuitous pathway axons take in the absence of a functional ROBO gene (3, 4). Human ROBO3 is a type I transmembrane glycoprotein that is synthesized as a 1386 amino acid (aa) precursor. It contains a 20 aa signal sequence, an 871 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 474 aa cytoplasmic region (5, 6). The ECD contains five C2-type Ig-like domains (aa 64-531) and three fibronectin (FN) type III domains (aa 555-863). The cytoplasmic region contains three of four possible 15-20 aa long CC (conserved cytoplasmic) motifs that are found in ROBO-1 (7, 8). Human ROBO3 has multiple isoforms. An alternate start site generates a 1365 aa A isoform and a 1341 aa B isoform. These two mature forms differ only over the first 26 and 2 amino acids of the N-terminus, respectively. There are multiple point mutations and insertions in the ROBO3 gene. Three result in truncated forms. One is 456 aa in length and ends after the Ig-like domain #4. A second is 770 aa in length and ends in the third FN domain. A third isoform is truncated after aa 1108 in the cytoplasmic region after CC2. At least one alternate splice form is also reported. It shows a 10 aa substitution between aa 1025 - 1034, followed by truncation. Human ROBO3 ECD is 84% and 91% aa identical to the ECD in mouse and canine ROBO3, respectively. Normally, axons originating on one side of the spinal cord are inhibited from crossing to the other side by a SLIT2-ROBO1 interaction at the midline. ROBO3 is permissive for this event. It is unclear how this is accomplished. One possibility is that it binds directly to ROBO1, blocking SLIT activation. A second possibility involves ROBO3 binding to SLIT2 in a nonproductive interaction. However, only ROBO3 Form B is known to bind to SLIT2 (9-11).
References
- Rajagopalan, S. et al. (2000) Neuron 28:767.
- Guthrie, S. (2004) Curr. Biol. 14:R632.
- Guthrie, S. (2001) Curr. Biol. 11:R300.
- Seeger, M. et al. (1993) Neuron 10:409.
- Jen, J.C. et al. (2004) Science 304:1509.
- Swiss-Prot. Accession # Q96MS0.
- Bashaw, G.J. et al. (2000) Cell 101:703.
- Kidd, T. et al. (1998) Cell 92:205.
- Camurri, L. et al. (2005) Mol. Cell. Neurosci. 30:485.
- Sabatier, C. et al. (2004) Cell 117:157.
- Mambetisaeva, E.T. et al. (2005) Dev. Dyn. 233:41.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional ROBO3 Products
Product Documents for Recombinant Human ROBO3 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human ROBO3 Fc Chimera Protein, CF
For research use only