Recombinant Human ROR1 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI9490

Biotinylated
R&D Systems, part of Bio-Techne
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AVI9490-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

Q01973

Structure / Form

Disulfide-linked homodimer

Conjugate

Biotin

Applications

Bioactivity

View all ROR1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human ROR1 protein
Human ROR1
(Ala24-Glu403)
Accession # Q01973		 IEGRMD Human IgG1
(Pro100-Lys330) 		 GG  Avi-tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala24

Predicted Molecular Mass

71 kDa

SDS-PAGE

85-105 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Human ROR1 Antibody (Catalog # MAB20001) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ROR1 Fc Chimera Avi-tag (Catalog # AVI9490) binds with an ED50 of 0.6-3.6 ng/mL.

Measured by its binding ability in a functional ELISA.
When Recombinant Human/Mouse Wnt-5a Recombinant Human/Mouse Wnt‑5a (Catalog # 645-WN) is immobilized at 4 µg/mL (100 µL/well), Biotinylated Recombinant Human ROR1 Fc Chimera Avi-tag (Catalog # AVI9490) binds with an ED50 of 5-40 μg/mL.

Scientific Data Images for Recombinant Human ROR1 Fc Chimera Avi-tag Protein, CF

Recombinant Human ROR1 Fc Chimera Avi-tag Protein Binding Activity

Recombinant Human ROR1 Fc Chimera Avi-tag Protein Binding Activity

When Human ROR1 Antibody (Catalog # MAB20001) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ROR1 Fc Chimera Avi-tag (Catalog # AVI9490) binds with an ED50 of 0.6-3.6 ng/mL.
Recombinant Human ROR1 Fc Chimera Avi-tag Protein SDS-PAGE

Recombinant Human ROR1 Fc Chimera Avi-tag Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human ROR1 Fc Chimera Avi-tag (Catalog # AVI9490) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-105 kDa and 170-210 kDa, respectively.

Formulation, Preparation and Storage

AVI9490
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROR1

ROR1 (Receptor tyrosine kinase-like orphan receptor 1), also known as neurotrophic tyrosine kinase receptor-related 1 (NTRKR1),  is a member of  the ROR family within receptor tyrosine kinases (RTK) superfamily. Two ROR family members (ROR1 and ROR2) have been identified and are characterized by the intracellular tyrosine kinase domains, highly related to those of the Trk-family receptor tyrosine kinases, and by the extracellular Frizzled-like cysteine-rich domains and kringle domains, which are common to receptors of the Wnt family members (1, 2). Human ROR1 is a type I transmembrane protein with 937 amino acids (aa) in length. It contains a 29 aa signal sequence, a 377 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 510 aa cytoplasmic region. Human ROR1 shares 97% and 58% aa sequence identity with mouse ROR1 and human ROR2, respectively (2, 3). ROR1 has been shown to play crucial roles in developmental morphogenesis by acting as receptors or co-receptors to mediate Wnt5a-induced signaling (4). The bioactivity of ROR1 is measured by its ability to bind biotinylated recombinant mouse Wnt-5a in a functional ELISA.

References

  1. Aghebati-Maleki, L. et al. (2017) Biomed. Pharmacother. 88:814.
  2. Borcherding N. et al. (2014) Protein Cell. 5:496.
  3. Masiakowski P. and Carroll R.D. (1992) J. Biol. Chem. 267:26181.
  4. Fukuda T. et al. (2008) Proc. Natl. Acad. Sci. U.S.A.  105:3047.

Long Name

Receptor Tyrosine Kinase-like Orphan Receptor 1

Alternate Names

NTRKR1

Entrez Gene IDs

4919 (Human); 26563 (Mouse)

Gene Symbol

ROR1

UniProt

Q01973

Additional ROR1 Products

Product Documents for Recombinant Human ROR1 Fc Chimera Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human ROR1 Fc Chimera Avi-tag Protein, CF

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