Recombinant Human ROR2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8609-RO

R&D Systems, part of Bio-Techne
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8609-RO-050
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Key Product Details

Source

NS0

Accession #

AAI30523

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all ROR2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human ROR2 protein
Human ROR2
(Val28-Gly403)
Accession # AAI30523		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val28

Predicted Molecular Mass

69 kDa

SDS-PAGE

80-92 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Measured by its ability to bind biotinylated Recombinant human/mouse Wnt-5a in a functional ELISA. The ED50 for this effect is 1.5-9 µg/mL.

Formulation, Preparation and Storage

8609-RO
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROR2

ROR2 (Receptor Tyrosine Kinase-like Orphan Receptor 2) is a member of the ROR family of receptor tyrosine kinases and is important for skeletal development, including bone and cartilage formation, as well as for the development of the central nervous system (1-5). Mature human ROR2 contains a 369 amino acid (aa) extracellular domain (ECD) and a 518 aa cytoplasmic tail containing an tyrosine kinase domain. The ROR2 ECD features an immunoglobulin domain, a cysteine-rich domain that resembles the Wnt-binding sites of Frizzled proteins, and a kringle domain. The ECD of human ROR2 shares 93% and 94% aa sequence identity with the ECD of mouse and rat ROR2, respectively. ROR2 binds the Wnt family ligand, Wnt-5a, to activate non-canonical Wnt signaling pathways (6-8). ROR2 is broadly expressed during embryonic development and can be found in cells of all three germ layers as well as in most organ tissues (9, 10). Activation of ROR2 signaling promotes cellular proliferation, differentiation, cell-polarization, and migration. ROR2 is important for osteogenic and chondrogenic differentiation of mesenchymal stem cells (11, 12). Mutations in ROR2 are associated with the skeletal disorders, brachydactyly type B and Robinow syndrome (6). ROR2 is also involved in neurite outgrowth and synapse development in the brain (4). A variety of tumors have been found to over-express ROR2. Depending upon the cellular context, ROR2 can act as a tumor suppressor or promoter (13). Expression of ROR2 within carcinomas is correlated with a poor prognosis of survival and increased rates of recurrence (14-16).

References

  1. DeChiara, T.M. et al. (2000) Nat. Genet. 24:271.
  2. Petrova, I.M. et al. (2014) Mol. Neurobiol. 49:303.
  3. Takeuchi, S. et al. (2000) Genes Cells 5:71.
  4. Paganoni, S. and A. Ferreira (2003) J. Neurosci. Res. 73:429.
  5. Endo, M. et al. (2012) J. Cell. Sci. 125:2017.
  6. Minami, Y. et al. (2010) Dev. Dyn. 239:1.
  7. Oishi, I. et al. (2003) Genes Cells 8:645.
  8. Wallkamm, V. et al. (2014) PLoS ONE 9:e109428.
  9. Al-Shawi, R. et al. (2001) Dev. Genes. Evol. 211:161.
  10. Matsuda, T. et al. (2001) Mech. Dev. 105:153.
  11. Cai, S.X. et al. (2014) J. Cell. Physiol. 229:791.
  12. Xin, H. et al. (2013) Int. J. Mol. Med. 31:583.
  13. Ford, C.E. et al. (2013) Int. J. Cancer 133:779.
  14. Rasmussen, N.R. et al. (2014) PLoS ONE 9:e116101.
  15. Mei, H. et al. (2014) Biochem. Biophys. Res. Commun. 453:703.
  16. Lu, B.J. et al. (2012) Mol. Med. Rep. 5:1033.

Long Name

Receptor Tyrosine Kinase-like Orphan Receptor 2

Alternate Names

BDB1, NTRKR2

Entrez Gene IDs

4920 (Human); 26564 (Mouse)

Gene Symbol

ROR2

UniProt

AAI30523

Additional ROR2 Products

Product Documents for Recombinant Human ROR2 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human ROR2 Fc Chimera Protein, CF

For research use only

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