Recombinant Human Ryk Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4907-RK

R&D Systems, part of Bio-Techne
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4907-RK-050
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Key Product Details

Source

CHO

Accession #

NP_001005861

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Ryk Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Ryk protein
Human Ryk
(Ala46-Thr224)
Accession # NP_001005861		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala46

Predicted Molecular Mass

46.7 kDa (monomer)

SDS-PAGE

60-70 kDa, reducing conditions

Activity

Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a functional ELISA with an estimated KD <50 nM.

Formulation, Preparation and Storage

4907-RK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ryk

Ryk (Related to tyrosine kinase) is an 70-90 kDa type I transmembrane glycoprotein that is an atypical member of the receptor tyrosine kinase superfamily (1, 2). Human Ryk cDNA encodes 604 amino acids (aa) that include a 25 aa signal sequence, a 199 aa extracellular domain (ECD, aa 26‑224) that contains a WIF (Wnt Inhibitory Factor) domain (aa 63‑191), a transmembrane sequence, and a cytoplasmic region with a nonfunctional Ser/Thr protein kinase domain (aa 327‑600) (1‑3). The cytoplasmic region participates in signaling by undergoing gamma‑secretase cleavage and translocating to the nucleus in response to Wnts (4). An isoform with a 31 aa substitution between aa 18‑46 is reported. Within the ECD, human Ryk shares approximately 94%, 92%, 86% and 81% aa sequence identity with mouse, rat, equine and canine Ryk, respectively. Ryk protein expression is reported in developing neurons of the corticospinal tract, ventral zone, and retina, as well as adult tissue epithelia, stroma and blood vessels (4‑9). Ryk binds Wnts and EphB2/B3 receptors via its WIF domain (8‑10). It may form a Wnt receptor complex with Frizzled receptors, serving as a link between Wnt and Dishevelled and converting Wnt/Frizzled attraction signals to repulsion signals (1, 6‑8, 10). In particular, Ryk is required for Wnt5a‑induced axon guidance after cortical axons cross the corpus callosum, and for Wnt3-mediated guidance of developing retinal ganglion cells (7, 8). Mice deleted for the Ryk gene show defects in axon guidance that produce craniofacial defects and shortened limbs (10, 11). Ryk-/- mice share a cleft palate phenotype with EphB2/B3-deleted mice (10). Ryk is often overexpressed in ovarian cancer (5).

References

  1. Stacker, S.A. et al. (1993) Oncogene 8:1347.
  2. Clark, C.E.J. et al. (2012) Neurosignals 20:202.
  3. Katso, R.M. et al. (1999) Mol. Cell. Biol. 19:6427.
  4. Lyu, J. et al. (2008) Dev. Cell 15:773.
  5. Katso, R.M. et al. (1999) Cancer Res. 59:2265.
  6. Liu, Y. et al. (2005) Nat. Neurosci. 8:1151.
  7. Schmitt, A.M. et al. (2006) Nature 439:31.
  8. Keeble, T.R. et al. (2006) J. Neurosci. 26:5840.
  9. Kamitori, K. et al. (2005) Biochem. Biophys. Res. Commun. 330:446.
  10. Lu, W. et al. (2004) Cell 119:97.
  11. Halford, M.M. et al. (2000) Nat. Genet. 25:414.

Long Name

Receptor Type Tyrosine Protein Kinase RYK

Alternate Names

JTK5

Entrez Gene IDs

6259 (Human); 20187 (Mouse); 140585 (Rat)

Gene Symbol

RYK

UniProt

NP_001005861

Additional Ryk Products

Product Documents for Recombinant Human Ryk Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human Ryk Fc Chimera Protein, CF

For research use only

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