Recombinant Human S100A7 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9085-SA

R&D Systems, part of Bio-Techne
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9085-SA-050
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Key Product Details

Source

E. coli

Accession #

AAH34687

Structure / Form

Noncovalently-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all S100A7 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human S100A7 protein
Ser2-Gln101 with an N-terminal Met

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser2 (Major) & Met1 (Minor)

Predicted Molecular Mass

11 kDa

SDS-PAGE

11 kDa, reducing conditions

Activity

Measured by its ability to induce IL-8 secretion in A431 human epithelial carcinoma cells.
The ED50 for this effect is 2-10 μg/mL.

Scientific Data Images for Recombinant Human S100A7 Protein, CF

Recombinant Human S100A7 Protein Bioactivity

Recombinant Human S100A7 Protein Bioactivity

Recombinant Human S100A7 (Catalog # 9085-SA) induces IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 2-10 μg/mL.

Formulation, Preparation and Storage

9085-SA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A7

S100A7, also known as Psoriasin, is a 11-12 kDa member of the S100 family of EF hand calcium binding proteins (1). Human S100A7 shares 32% amino acid sequence identity with mouse S100A7A, the closest related protein in mouse (2). It is acetylated at the N-terminus and binds both calcium and zinc ions (3, 4). S100A7 is up-regulated in keratinocytes of psoriasis and atopic dermatitis lesions (5-9), as well as in epithelial cells of the tongue, eye, and female genital tract (10-12). Its up-regulation can be induced by bacterial exposure, inflammatory cytokines, or epidermal barrier disruption (3, 9, 11, 12). S100A7 supports epithelial integrity through killing E. coli by sequestration of zinc (3, 5) and through inducing the up-regulation of tight junction proteins (13). The interaction of S100A7 with RAGE promotes the migration of immune cells and the infiltration of macrophages into tumor sites (7, 14).

References

  1. Donato, R. et al. (2013) Curr. Mol. Med. 13:24.
  2. Madsen, P. et al. (1991) J. Invest. Dermatol. 97:701.
  3. Glaser, R. et al. (2005) Nat. Immunol. 6:57.
  4. Brodersen, D.E. et al. (1999) Biochemistry 38:1695.
  5. Lee, K.C. and R.L. Eckert (2007) J. Invest. Dermatol. 127:945.
  6. Anderson, K.S. et al. (2009) Br. J. Dermatol. 160:325.
  7. Wolf, R. et al. (2008) J. Immunol. 181:1499.
  8. Al-Haddad, S. et al. (1999) Am. J. Pathol. 155:2057.
  9. Glaser, R. et al. (2009) J. Invest. Dermatol. 129:641.
  10. Meyer, J.E. et al. (2008) Mucosal Immunol. 1:239.
  11. Garreis, F. et al. (2011) Invest. Opthalmol. Vis. Sci. 52:4914.
  12. Mildner, M. et al. (2010) Mucosal Immunol. 3:602.
  13. Hattori, F. et al. (2014) Br. J. Dermatol. 171:742.
  14. Nasser, M.W. et al. (2015) Cancer Res. 75:974.

Long Name

S100 Calcium Binding Protein A7

Alternate Names

PSOR1

Entrez Gene IDs

6278 (Human); 192344 (Mouse)

Gene Symbol

S100A7

UniProt

AAH34687

Additional S100A7 Products

Product Documents for Recombinant Human S100A7 Protein, CF

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Product Specific Notices for Recombinant Human S100A7 Protein, CF

For research use only

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