Recombinant Human Serpin B2/PAI-2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9206-PI

R&D Systems, part of Bio-Techne
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9206-PI-025
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Key Product Details

Source

E. coli

Accession #

P05120

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Serpin B2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Serpin B2 protein
Glu2-Ala65/Ala99-Pro415, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

44 kDa

SDS-PAGE

41 kDa, reducing conditions

Activity

Measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC).

The inhibition IC50 is <15 nM, as measured under the described conditions.

Formulation, Preparation and Storage

9206-PI
Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Chaps.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Serpin B2

Serpin B2, also known as PAI-2, is an approximately 60 kDa serine protease inhibitor (1). It is primarily secreted by macrophages and monocytes (2, 3) and can form disulfide-linked multimers (4, 5). Serpin B2 inhibits both the urokinase-type and tissue-type plasminogen activators (uPA and tPA) (3, 4, 6). Serpin B2 also promotes the clearance of uPA by enhancing its binding and uptake by LRP (7). It limits fibril formation by Huntington protein (HTT) and beta-Amyloid peptides (8). It promotes Th2 biased immune responses and is important for intestinal CCL2 production, monocyte recruitment, and nematode clearance (2, 9). A non-glycosylated form of Serpin B2 is retained intracellularly where it interferes with TNF-a induced apoptosis by protecting the Retinoblastoma protein (RB1) from calpain digestion (10). It also inhibits proteasome activity in activated endothelial cells (11). Serpin B2 is upregulated in HIV-1 infected PBMC, induces RB1 upregulation, and promotes HIV-1 replication (12). Human Serpin B2 shares 76% and 73% amino acid sequence identity with mouse and rat Serpin B2, respectively.

References

  1. Shea-Donohue, T. et al. (2014) Gut Microbes 5:254.
  2. Schroder, W.A. et al. (2010) J. Immunol. 184:2663.
  3. Ritchie, H. et al. (1999) Thomb. Haemost. 81:96.
  4. Mikus, P. et al. (1993) Eur. J. Biochem. 218:1071.
  5. Wilczynska, M. et al. (2003) EMBO J. 22:1753.
  6. Baker, M.S. et al. (1990) Cancer Res. 50:4676.
  7. Croucher, D. et al. (2006) J. Biol. Chem. 281:10206.
  8. Lee, J.A. et al. (2015) PLoS One 10:e0130136.
  9. Zhao, A. et al. (2013) J. Immunol. 190:5779.
  10. Tonnetti, L. et al. (2008) Cancer Res. 68:5648.
  11. Boncela, J. et al. (2011) J. Biol. Chem. 286:43164.
  12. Darnell, G.A. et al. (2006) J. Biol. Chem. 281:31348.

Alternate Names

PAI2, PLANH2, Urokinase Inhibitor

Entrez Gene IDs

5055 (Human); 18788 (Mouse); 60325 (Rat)

Gene Symbol

SERPINB2

UniProt

P05120

Additional Serpin B2 Products

Product Documents for Recombinant Human Serpin B2/PAI-2 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Serpin B2/PAI-2 Protein, CF

For research use only

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