Recombinant Human Serpin B3/SSCA1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6528-PI

R&D Systems, part of Bio-Techne
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6528-PI-010
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

P29508

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Serpin B3/SCCA1 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Serpin B3/SCCA1 protein
Asn2-Pro390, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

45 kDa

SDS-PAGE

40-43 kDa, reducing conditions

Activity

Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC (Catalog # ES008).
The IC50 is <5 nM, as measured under the described conditions.

Formulation, Preparation and Storage

6528-PI
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Serpin B3/SCCA1

Serpin B3, also known as squamous cell carcinoma antigen-1 (SCCA-1), is a member of the serpin superfamily of serine protease inhibitors (1). Serpins primarily inhibit serine proteases and some are known to inhibit caspases and papain-like cysteine proteases (1). Serpin B3 belongs to the subgroup ovalbumin-related serpins which are involved in the regulation of apoptosis, inflammation, angiogenesis and embryogenesis (2). Serpin B3 was initially isolated from human cervical squamous cell tissue. It is a tumor marker for squamous cell carcinomas in various areas including the cervix, head, neck and lung (3). Unlike typical serpins, Serpin B3 shows inhibitory activity against papain-like lysosomal cysteine proteases Cathepsin K, L, and S, while lacking inhibitory activity against serine proteases (4).

References

  1. Law, R. et al. (2006) Genome Biol. 7:216.
  2. Benarafa, C. and Remold-O’Donnell, E. (2005) Proc. Natl. Acad. Sci. 102:11367.
  3. Higgens, W.J. et al. (2010) J. Biol. Chem. 285:3722.
  4. Schick, C. et al. (1998) Biochemistry. 37:5258.

Alternate Names

SCCA1, T4-A

Entrez Gene IDs

6317 (Human)

Gene Symbol

SERPINB3

UniProt

P29508

Additional Serpin B3/SCCA1 Products

Product Documents for Recombinant Human Serpin B3/SSCA1 Protein, CF

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Product Specific Notices for Recombinant Human Serpin B3/SSCA1 Protein, CF

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