Recombinant Human Serpin D1/Heparin Cofactor II Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3198-PI
Key Product Details
Source
Sf 21 (stably transfected)
Accession #
Conjugate
Unconjugated
Applications
Inhibition Activity
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (stably transfected)-derived human Serpin D1/Heparin Cofactor II protein
Gly20-Ser499, with a C-terminal 10-His tag
Gly20-Ser499, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly20
Predicted Molecular Mass
56 kDa
SDS-PAGE
62 kDa, reducing conditions
Formulation, Preparation and Storage
3198-PI
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Serpin D1/Heparin Cofactor II
Serpin D1, also known as Heparin Cofactor II, is a member of the Serpin superfamily of the serine protease inhibitors (1). Similar to Serpins A5 and C1, it inhibits thrombin and this activity is enhanced by heparin. Interestingly, a C-terminal His-tagged recombinant Serpin D1 had enhanced heparin effect, which was maintained in a plasma-based thrombin inhibition assay (2). Congenital Serpin D1 deficiency is a potential risk factor for thrombosis (3). The full‑length human cDNA was expressed and the purified protein corresponded to the secreted form with anti‑thrombin activity.
References
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Bauman, S.J. et al. (1999) J. Biol. Chem. 274:34556.
- Tollefsen, D.M. (2002) Arch. Pathol. Lab. Med. 126:1394.
Alternate Names
HC2, HCF2, Heparin Cofactor II, LS2
Gene Symbol
SERPIND1
UniProt
Additional Serpin D1/Heparin Cofactor II Products
Product Documents for Recombinant Human Serpin D1/Heparin Cofactor II Protein, CF
Product Specific Notices for Recombinant Human Serpin D1/Heparin Cofactor II Protein, CF
For research use only
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