Recombinant Human sFRP-1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5396-SF

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
5396-SF-025
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

AAB70793

Conjugate

Unconjugated

Applications

Bioactivity

View all sFRP-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human sFRP-1 protein
Ser32-Lys314

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser32, Phe39 (minor) & Asp42 (minor)

Predicted Molecular Mass

32.6 kDa

SDS-PAGE

33-40 kDa, reducing conditions

Activity

Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. Ko, L. et al. (2002) Exp. Cell Res. 280:280.
The ED50 for this effect is 0.15-0.75 µg/mL.

Formulation, Preparation and Storage

5396-SF
Formulation Lyophilized from a 0.2 μm filtered solution in Phosphate and NaCl.
Reconstitution
Reconstitute at 250 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: sFRP-1

Secreted Frizzled Related Protein-1 (sFRP-1), also known as SARP-2, FRP, and FrzA, belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled transmembrane Wnt receptors. The sFRP proteins are approximately 30-35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-3). Mature human sFRP-1 shares 97% and 96% amino acid sequence identity with mouse and rat sFRP-1, respectively. It binds to Wnt-1, -2, -3a, and -4 and prevents their activation of canonical beta-catenin signaling (4-7). sFRP-1 is up-regulated in the ischemic heart where it limits inflammation and the severity of tissue damage following myocardial infarction (8-10). During angiogenesis it induces endothelial cell migration, tube formation, and vessel formation (11). sFRP-1 is expressed by bone marrow stromal cells and osteoclasts (12). It binds to TRANCE/RANK Ligand, inhibits osteoclast and osteoblast formation and survival, and inhibits the formation of trabecular bone (13, 14). sFRP-1 additionally restricts kidney tubule formation (7), the inflammatory activation of leukocytes (10), early B lymphoid progression (12), and vitreous fluid outflow in the eye (15).

References

  1. Cruciat, C.M. and C. Niehrs (2013) Cold Spring Harb. Perspect. Biol. 5:e015081.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Finch, P.W. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6770.
  4. Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.
  5. Dennis, S. et al. (1999) J. Cell Sci. 112:3815.
  6. Wawrzak, D. et al. (2007) Biochem. Biophys. Res. Commun. 357:1119.
  7. Yoshino, K. et al. (2001) Mech. Dev. 102:45.
  8. Barandon, L. et al. (2002) Circulation 108:2282.
  9. Sklepkiewicz, P. et al. (2015) Circ. Heart Fail. 8:362.
  10. Barandon, L. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:e80.
  11. Dufourcq, P. et al. (2002) Circ. Res. 106:3097.
  12. Yokota, T. et al. (2008) J. Immunol. 181:6061.
  13. Hausler, K.D. et al. (2004) J. Bone Miner. Res. 19:1873.
  14. Bodine, P.V.N. et al. (2004) Mol. Endocrinol. 18:1222.
  15. Wang, W.-H. et al. (2008) J. Clin. Invest. 118:1056.

Long Name

Secreted Frizzled Related Protein 1

Alternate Names

FrzA, SARP-2, sFRP1

Entrez Gene IDs

6422 (Human); 20377 (Mouse); 84402 (Rat)

Gene Symbol

SFRP1

UniProt

AAB70793

Additional sFRP-1 Products

Product Documents for Recombinant Human sFRP-1 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human sFRP-1 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.