Recombinant Human sFRP-3 Protein

R&D Systems, part of Bio-Techne | Catalog # 7584-SF

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Carrier Free
7584-SF-025/CF
Catalog #
Availability
Size / Price
Qty
With Carrier
7584-SF-025
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

AAB51298

Conjugate

Unconjugated

Applications

Bioactivity

View all sFRP-3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human sFRP-3 protein
Ala33-Asn325

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala33

Predicted Molecular Mass

33.2 kDa

SDS-PAGE

39 kDa, reducing conditions

Activity

Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. Ko, L. et al. (2002) Exp. Cell Res. 280:280.
The ED50 for this effect is 0.05-0.25 μg/mL.

Reviewed Applications

Read 1 review rated 5 using 7584-SF in the following applications:

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 7584-SF
Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 7584-SF/CF
Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: sFRP-3

Secreted Frizzled Related Protein‑3 (sFRP‑3), also known as FRZB, belongs to a family of Wnt-binding proteins with homology to the ligand‑binding domain of the Frizzled receptors. sFRPs are approximately 30‑35 kDa in size and contain an N‑terminal Frizzled‑like domain with 10 conserved cysteines and a Netrin-like C‑terminal domain (1‑4). Mature human sFRP‑3 shares 93% aa sequence identity with mouse and rat sFRP‑3. It neutralizes the bioactivity of Wnt‑1, ‑5a, ‑9a, Xenopus Xwnt‑8, and EGF (5‑8). sFRP‑3 regulates somitic myogenesis and osteoblast differentiation as well as morphogenesis of the cochlear epithelium, hair follicle, and cardiac atrioventricular cushion (6, 8‑10). sFRP‑3 also functions as a tumor suppressor in a variety of cancers including melanoma, medulloblastoma, soft tissue sarcoma, and androgen‑independent prostate cancer. It inhibits epithelial-mesenchymal transition, tumor growth and invasion, and the activity of MMP‑2 and MMP‑9 (7, 11‑13). In contrast, it can promote tumor growth and angiogenesis in renal cancer (14).

References

  1. Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. USA 94:2859.
  4. Hoang, B. et al. (1996) J. Biol. Chem. 271:26131.
  5. Wang, S. et al. (1997) Cell 88:757.
  6. Person, A.D. et al. (2005) Dev. Biol. 278:35.
  7. Ekstrom, E.J. et al. (2011) PLoS ONE 6:e18674.
  8. Scardigli, R. et al. (2008) PLoS ONE 3:e2471.
  9. Qian, D. et al. (2007) Dev. Biol. 306:121.
  10. Chung, Y.-S. et al. (2004) J. Bone Miner. Res. 19:1395.
  11. Kongkham, P.N. et al. (2010) Oncogene 29:3017.
  12. Guo, Y. et al. (2008) Cancer Res. 68:3350.
  13. Zi, X. et al. (2005) Cancer Res. 65:9762.
  14. Hirata, H. et al. (2010) Cancer Res. 70:1896.

Long Name

Secreted Frizzled Related Protein 3

Alternate Names

Fritz, FRZB, Frzb1, sFRP3

Entrez Gene IDs

2487 (Human); 20378 (Mouse)

Gene Symbol

FRZB

UniProt

AAB51298

Additional sFRP-3 Products

Product Documents for Recombinant Human sFRP-3 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 7584-SF (with carrier)
Download SDS 7584-SF/CF (carrier free)

Product Specific Notices for Recombinant Human sFRP-3 Protein

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.