Recombinant Human Sirtuin 2/SIRT2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4358-DA
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Sirtuin 2/SIRT2 protein
Met1-Gln389, with a C-terminal 6-His tag
Met1-Gln389, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No results obtained: Presumed blocked
Predicted Molecular Mass
44 kDa
SDS-PAGE
43-49 kDa, reducing conditions
Activity
Measured by its ability to remove the acetyl group from a fluorogenic peptide substrate Ac-RGK(Ac)-AMC (Catalog # ES016) in a coupled assay.
The specific activity is >3 pmol/min/µg, as measured under the described conditions.
The specific activity is >3 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
4358-DA
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Sirtuin 2/SIRT2
Sirtuin 2, encoded by the SIRT2 gene, is also known as SIR2 (silent information regulator 2)-like protein 2. It is a nicotinamide adenine dinucleotide (NAD)-dependent histone/protein deacetylase (1, 2). The SIR2 family of enzymes is classified as class III histone deacetylases (HDACs) and has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging (3, 4). Unlike class I and class II HDACs, the enzymatic activity of class III HDACs is NAD dependent and insensitive to HDAC inhibitor trichostatin A (5).
References
- Frye, R.A. (1999) Biochem. Biophys. Res. Commun. 260:273.
- North, B.J. et al. (2003) Mol. Cell. 11:437.
- North, B.J. and Verdin, E. (2004) Genome Biol. 5:224.
- Marmorstein, R. (2004) Biochem. Soc. Trans. 32:904.
- Gray, S.G. and Ekstrom, T.J. (2001) Exp. Cell Res. 262:75.
Long Name
Silent Mating Type Information Regulation 2 Homolog
Alternate Names
SIR2L2, SIRT2
Gene Symbol
SIRT2
UniProt
Additional Sirtuin 2/SIRT2 Products
Product Documents for Recombinant Human Sirtuin 2/SIRT2 Protein, CF
Product Specific Notices for Recombinant Human Sirtuin 2/SIRT2 Protein, CF
For research use only
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