Recombinant Human Slit3 (aa 1120-1523) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9067-SL

R&D Systems, part of Bio-Techne
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9067-SL-050
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Key Product Details

Source

HEK293

Accession #

O75094

Conjugate

Unconjugated

Applications

Bioactivity

View all Slit3 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Slit3 protein
Thr1120-Ser1523, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr1120

Predicted Molecular Mass

45 kDa

SDS-PAGE

56-63 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human Slit3, immobilized at 1.25-2.5 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.

Scientific Data Images for Recombinant Human Slit3 (aa 1120-1523) Protein, CF

Recombinant Human Slit3 (aa 1120-1523) Protein Bioactivity

Recombinant Human Slit3 (aa 1120-1523) Protein Bioactivity

Recombinant Human Slit3 (Catalog # 9067-SL) Induces Cortical Neurite Outgrowth. A) Untreated E16-E18 embryonic rat cortical neurons. B) Neurite outgrowth in E16-E18 embryonic rat cortical neurons treated with 1.25 µg/ml of Recombinant Human Slit3.

Formulation, Preparation and Storage

9067-SL
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Slit3

Slit3 is an approximately 200 kDa member of the Slit family of large secreted axon guidance molecules that are ligands for ROBO receptors (1, 2). It is secreted to the extracellular space and also localizes in mitochondria via a mitochondria localization sequence (3). Mature human Slit3 consists of 4 cassettes of leucine-rich repeats (LRR) flanked by LRR N-terminal and C-terminal domains, followed by multiple EGF-like domains, a Laminin G-like domain, and a C-terminal cysteine-rich domain. Alternative splicing generates additional isoforms with a substituted cysteine-rich domain or a deletion in the last EGF-like domain and Laminin G-like domain. During development, Slit3 is expressed in the ventral neural tube, developing sensory organs, limb buds, and developing areas of the limbs in patterns that overlap with but are discrete from Slit1 and Slit2 (1, 2, 4). Axons will not be allowed to recross the floor plate unless all three Slit genes are disrupted, suggesting some overlap in Slit function (5). Slit3 is also expressed in the lung, kidney, skeletal muscle, and heart, both during development and postnatally (6-8). Mice with genetically disrupted Slit3 show abnormalities in diaphragm and kidney development (7, 8). Slit3 is additionally expressed by vascular endothelial cells and smooth muscle cells (9). It binds to both ROBO1 and ROBO4, but it is the ROBO4 interaction that mediates Slit3-induced angiogenesis (9). It also can enhance the chemokine-induced migration of monocytes (10). The related Slit2 protein is cleaved in vivo (at a site conserved in Slit3), and the resulting C-terminal fragment binds Plexin A1 and retains the ability to repel axon migration (4, 11). The corresponding C-terminal fragment of Slit3 is able to bind heparin and neutralize its anti-coagulant activity (12). Within this fragment (aa 1120-1523), human Slit3 shares 93% amino acid identity with mouse and rat Slit3.

References

  1. Blockus, H. and A. Chedotal (2014) Curr. Opin Neurobiol. 27:82.
  2. Gara, R.K. et al. (2015) Drug Discov. Today 20:156.
  3. Little, M. H. et al. (2001) Am. J. Physiol. Cell Physiol. 281:C486.
  4. Brose, K. et al. (1999) Cell 96:795.
  5. Long, H. et al. (2004) Neuron 42:213.
  6. Greenberg, J.M. et al. (2004) Dev. Dyn. 230:350.
  7. Liu, J. et al. (2003) Mech. Dev. 120:1059.
  8. Yuan, W. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5217.
  9. Zhang, B. et al. (2009) Blood 114:4300.
  10. Geutskens, S.B. et al. (2010) J. Immunol. 185:7691.
  11. Delloye-Bourgeois, C. et al. (2015) Nat. Neurosci. 18:36.
  12. Condac, E. et al. (2012) Glycobiology 22:1183.

Long Name

SLIT Homolog 3

Alternate Names

MEGF5, SLIL2

Entrez Gene IDs

6586 (Human); 20564 (Mouse); 83467 (Rat)

Gene Symbol

SLIT3

UniProt

O75094

Additional Slit3 Products

Product Documents for Recombinant Human Slit3 (aa 1120-1523) Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human Slit3 (aa 1120-1523) Protein, CF

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