Recombinant Human SMOC-2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 5140-SM
Key Product Details
Product Specifications
Source
Gln22-Gly446, with a C-terminal 6-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
When 5 x 104 cells/well are added to rhSMOC-2 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37 °C. The ED50 for this effect is 0.4-1.6 μg/mL.
Formulation, Preparation and Storage
5140-SM
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution |
Reconstitute at 200 μg/mL in PBS.
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Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: SMOC-2
SMOC-2 (secreted, or SPARC-related, modular calcium-binding protein 2), previously called SMAP2 (smooth muscle-associated protein 2), is a 55 kDa glycoprotein that is a member of the SPARC family of matricellular proteins (1, 2). The human SMOC-2 cDNA encodes 446 amino acids (aa), including a 21 aa signal sequence, a Kazal-like domain (aa 40 - 84), two thyroglobulin type-1 segments (aa 87 - 153 and 213 - 281) and two EF-hand sequences (aa 347 - 382 and 384 - 419) (1, 2). Of three splice variants, one shows a 13 aa substitution for aa 442 - 446, another shows an 11 aa insertion after Thr170, and a third contains both of these variations. Mature human SMOC-2 shares 95%, 95% and 94% aa identity with mouse, rat and canine SMOC-2, respectively. SMOC-2 is widely expressed in the extracellular matrix and appears to have adhesion-related functions (1 - 5). Recombinant bacterially produced human SMOC-1 and SMOC-2 can bind the acute phase protein, C-reactive protein, and the adhesion proteins, fibulin and vitronectin, while keratinocyte SMOC-2 binds integrins alphav beta1 and alphav beta6 (3, 6). SMOC-2 promotes cell cycle progression by signaling through the integrin-linked kinase (ILK) to upregulate cyclin-D1 (4). When expressed in the endothelial extracellular matrix, it potentiates growth factor-induced angiogenesis (5). SMOC-2 expression is upregulated during neointima formation, promoting proliferation and migration of vascular smooth muscle (2). In the skin, it promotes keratinocyte attachment and migration (6). Since Kazal and thyroglobulin domains are often found in protease inhibitors, SMOC-2 is also proposed to inhibit proteases in the lung and artery (2, 7).
References
- Vannahme, C. et al. (2003) Biochem. J. 373:805.
- Nishimoto, S. et al. (2002) Biochim. Biophys. Acta 1576:225.
- Novinec, M. et al. (2008) Protein Expr. Purif. 62:75.
- Liu, P. et al. (2008) Mol. Biol. Cell 19:248.
- Rocnik, E.F. et al. (2006) J. Biol. Chem. 281:22855.
- Maier, S. et al. (2008) Exp. Cell Res. 314:2477.
- Wilk, J.B. et al. (2007) Am. J. Respir. Crit. Care Med. 175:554.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional SMOC-2 Products
Product Documents for Recombinant Human SMOC-2 Protein, CF
Product Specific Notices for Recombinant Human SMOC-2 Protein, CF
For research use only