Recombinant Human Sphingosine Kinase 1/SPHK1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5536-SK

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
5536-SK-010
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

Sf 21 (baculovirus)

Accession #

NP_068807

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Sphingosine Kinase 1/SPHK1 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Sphingosine Kinase 1/SPHK1 protein
Asp2-Leu398 with an N-terminal Met and 6-His tag

Purity

>75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Inconclusive result, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments.

Predicted Molecular Mass

45 kDa

SDS-PAGE

40-43 kDa, reducing conditions

Activity

Measured by its ability to phosphorylate Omega-(7-nitro-2-1,3-benzoxadiazol-4-yl)-D-erythro-sphingosine (NBD-sphingosine).
The specific activity is >200 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

5536-SK
Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl, Glycerol, DTT and Triton®.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Sphingosine Kinase 1/SPHK1

Sphingosine kinases are cytosolic or membrane‑associated enzymes that catalyze the phosphorylation of sphingosine to sphingosine‑1‑phosphate (S1P). Two types of sphingosine kinases, SPHK1 and SPHK2, are known to be expressed in human cells. The two enzymes share considerable amino acid sequence similarity, but differ in their N‑terminal and central regions (1). The two proteins also differ in tissue distribution and some kinetic properties (1). S1P is a lipid messenger that regulates diverse physiological processes including cell proliferation, migration, apoptosis, inflammation, calcium homeostasis and cytoskeletal structure (2, 3). The level of S1P is tightly controlled by SPHKs and S1P degrading enzymes. SPHK1 and its activation can be stimulated by several growth factors such as tumor necrosis factor-alpha, epidermal growth factor and transforming growth factor-beta (3, 4). Expression of SPHK1 has been found to increase in many human solid tumors and overexpression of SPHK1 is associated with tumor angiogenesis (5). Such studies have implicated SPHK1 as a new target for cancer treatment.

References

  1. Liu, H. et al. (2000) J. Biol. Chem. 275:19513.
  2. Spiegel, S. (1999) J. Leukocyte Biol. 65:341.
  3. Alemany, R. et al. (2007) Naunyn-Schmiedegerg’s Arch. Pharmacol. 374:413.
  4. Pederson, L. et al. (2008) Proc. Natl. Acad. Scis USA. 105:20764.
  5. Shida, D. et al. (2008) Curr. Drug Targets. 9:662.

Alternate Names

SK1, SPK1

Entrez Gene IDs

8877 (Human); 20698 (Mouse); 170897 (Rat)

Gene Symbol

SPHK1

UniProt

NP_068807

Additional Sphingosine Kinase 1/SPHK1 Products

Product Documents for Recombinant Human Sphingosine Kinase 1/SPHK1 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Sphingosine Kinase 1/SPHK1 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.